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  • Primary Antibodies ›
  • cGKII Antibodies

Invitrogen

cGKII Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers

Cite cGKII Polyclonal Antibody

  • Antibody Testing Data (2)
cGKII Antibody in Western Blot (WB)
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cGKII Antibody in Western Blot (WB)
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cGKII Antibody (PA5-116863) in WB

Western blot analysis of cGKII in extracts of various cell lines. Samples were incubated with cGKII Polyclonal antibody (Product # PA5-116863) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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cGKII Antibody in Western Blot (WB)
cGKII Antibody in Immunoprecipitation (IP)
cGKII Polyclonal Antibody

Product Details

PA5-116863

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 400 µg-600 µg extracts of
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human PRKG2 (NP_0062501)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.22 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901494

Product Specific Information

Positive Samples: Mouse lung, Rat brain

Immunogen sequence: MGNGSVKPKH SKHPDGHSGN LTTDALRNKV TELERELRRK DAEIQEREYH LKELREQLSK QTVAIAELTE ELQNKCIQLN KLQDVVHMQG GSPLQASPDK VPLEVHRKTS GLVSLHSRRG AKAGVSAEPT TRTYDLNKPP EFSFEKARVR KDSSEKKLIT

Target Information

PRKG2 (PKG2, cGKII) exhibits many cellular functions and has cAMP- and cGMP-dependent protein kinase activity. cGKII is thought to play a key role in a diverse set of physiological pathway. cGKII may mediate intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin. Identification of the pathway that mediates intestinal fluid secretion by E. coli. cGKII is postulated to be a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CGK 2; cGK2; cGKII; cGMP dependent protein kinase type II; cGMP-dependent protein kinase 2; cGMP-dependent protein kinase II; EC 2.7.11.12; protein kinase cGMP- dependent type 2; protein kinase, cGMP- dependent, type 2; protein kinase, cGMP- dependent, type II; type II cGMP-dependent protein kinase

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Gene Aliases: AW212535; cGK-II; CGKII; GDPKII; Prkg2; Prkgr2

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UniProt ID: (Rat) Q64595

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Entrez Gene ID: (Rat) 25523, (Mouse) 19092

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Function(s)
cGMP-dependent protein kinase activity ATP binding cGMP binding protein homodimerization activity nucleotide binding protein kinase activity protein serine/threonine kinase activity protein binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
protein phosphorylation circadian rhythm circadian regulation of gene expression peptidyl-serine autophosphorylation protein targeting to plasma membrane phosphorylation
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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