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Proteintech

c-SRC Monoclonal Antibody (5E10C4)

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View all (75) SRC antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite c-SRC Monoclonal Antibody (5E10C4)

c-SRC Monoclonal Antibody (5E10C4)
  • Antibody Testing Data (10)
c-SRC Antibody in Immunocytochemistry (ICC/IF)
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c-SRC Antibody in Immunocytochemistry (ICC/IF)
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c-SRC Antibody (60315-1-IG) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 60315-1-IG (c-SRC antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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c-SRC Antibody in Immunocytochemistry (ICC/IF)
c-SRC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
c-SRC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
c-SRC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
c-SRC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
c-SRC Antibody in Western Blot (WB)
c-SRC Antibody in Western Blot (WB)
c-SRC Antibody in Western Blot (WB)
c-SRC Antibody in Western Blot (WB)
c-SRC Antibody in Western Blot (WB)

Product Details

60315-1-IG

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:8,000
View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400
-

Immunocytochemistry (ICC/IF)

1:50-1:500
View 1 publication 1 publication
Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5E10C4

Immunogen

c-SRC Fusion Protein Ag22306 (1-536 aa encoded by BC011566)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated
  • CoraLite 594
  • CoraLite Plus 488
  • Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGSNKSKPK DASQRRRSLE PAENVHGAGG GAFPASQTPS KPASADGHRG PSAAFAPAAA EPKLFGGFNS SDTVTSPQRA GPLAGGVTTF VALYDYESRT ETDLSFKKGE RLQIVNNTEG DWWLAHSLST GQTGYIPSNY VAPSDSIQAE EWYFGKITRR ESERLLLNAE NPRGTFLVRE SETTKGAYCL SVSDFDNAKG LNVKHYKIRK LDSGGFYITS RTQFNSLQQL VAYYSKHADG LCHRLTTVCP TSKPQTQGLA KDAWEIPRES LRLEVKLGQG CFGEVWMGTW NGTTRVAIKT LKPGTMSPEA FLQEAQVMKK LRHEKLVQLY AVVSEEPIYI VTEYMSKGSL LDFLKGETGK YLRLPQLVDM AAQIASGMAY VERMNYVHRD LRAANILVGE NLVCKVADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW SFGILLTELT TKGRVPYPGM VNREVLDQVE RGYRMPCPPE CPESLHDLMC QCWRKEPEER PTFEYLQAFL EDYFTSTEPQ YQPGENL (1-536 aa encoded by BC011566)

Target Information

SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: c-Src; EC 2.7.10.2; neuronal proto-oncogene tyrosine-protein kinase Src; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; pp60c-src; pp60src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; Rous sarcoma oncogene; RP5-823N20.1; tyrosine kinase pp60c-src; tyrosine protein kinase c-src; tyrosine protein kinase pp60-c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

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Gene Aliases: ASV; AW259666; c-SRC; p60-Src; pp60c-src; SRC; SRC1; THC6

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UniProt ID: (Human) P12931, (Mouse) P05480, (Rat) Q9WUD9

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Entrez Gene ID: (Human) 6714, (Chinese hamster) 100757188, (Mouse) 20779, (Rat) 83805

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Function(s)
protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity SH3/SH2 adaptor activity protein kinase C binding receptor binding insulin receptor binding integrin binding protein binding ATP binding protein C-terminus binding kinase activity enzyme binding kinase binding heme binding estrogen receptor binding SH2 domain binding ion channel binding ephrin receptor binding phosphoprotein binding hormone receptor binding growth factor receptor binding scaffold protein binding cadherin binding involved in cell-cell adhesion nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding protein domain specific binding protein complex binding cell adhesion molecule binding
Process(es)
immune system process protein phosphorylation cell cycle cell adhesion epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway cell proliferation positive regulation of gene expression regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of glucose metabolic process positive regulation of smooth muscle cell migration phosphorylation cell migration peptidyl-serine phosphorylation peptidyl-tyrosine phosphorylation regulation of cell-cell adhesion cell differentiation forebrain development positive regulation of protein autophosphorylation activation of protein kinase B activity negative regulation of protein homooligomerization regulation of intracellular estrogen receptor signaling pathway adherens junction organization substrate adhesion-dependent cell spreading cellular response to reactive oxygen species intracellular signal transduction osteoclast development cellular response to platelet-derived growth factor stimulus peptidyl-tyrosine autophosphorylation regulation of cell proliferation positive regulation of apoptotic process negative regulation of apoptotic process stress fiber assembly negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of protein binding positive regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity transcytosis innate immune response bone resorption positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cell adhesion negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of insulin receptor signaling pathway protein autophosphorylation neurotrophin TRK receptor signaling pathway oogenesis positive regulation of cytokine secretion progesterone receptor signaling pathway positive regulation of protein transport regulation of cell cycle negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization uterus development branching involved in mammary gland duct morphogenesis regulation of cell projection assembly positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to peptide hormone stimulus cellular response to progesterone stimulus cellular response to fluid shear stress cellular response to transforming growth factor beta stimulus positive regulation of podosome assembly angiotensin-activated signaling pathway involved in heart process positive regulation of canonical Wnt signaling pathway positive regulation of intracellular signal transduction positive regulation of lamellipodium morphogenesis positive regulation of DNA biosynthetic process regulation of early endosome to late endosome transport negative regulation of anoikis negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway regulation of caveolin-mediated endocytosis stimulatory C-type lectin receptor signaling pathway signal transduction signal complex assembly central nervous system development response to mechanical stimulus response to virus response to acidic pH positive regulation of platelet-derived growth factor receptor signaling pathway single organismal cell-cell adhesion platelet activation intracellular estrogen receptor signaling pathway T cell costimulation protein destabilization response to nutrient levels negative regulation of telomere maintenance via telomerase cellular response to insulin stimulus positive regulation of integrin activation Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway response to drug response to hydrogen peroxide regulation of vascular permeability regulation of bone resorption platelet-derived growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration positive regulation of small GTPase mediated signal transduction response to mineralocorticoid response to electrical stimulus negative regulation of telomerase activity cellular response to lipopolysaccharide cellular response to fatty acid cellular response to hypoxia regulation of podosome assembly positive regulation of protein serine/threonine kinase activity response to fatty acid
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