Bethyl Laboratories

c-Cbl Polyclonal Antibody

Name: c-Cbl Polyclonal Antibody
Brand: Bethyl Laboratories
SKU: A302-337A

View all (49) c-Cbl antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

FIGURE: 1 / 1

c-Cbl Antibody (A302-337A) in WB

Detection of human c-Cbl by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-c-Cbl antibody (Product # A302-337A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. c-Cbl was also immunoprecipitated by rabbit anti-c-Cbl antibody A302-338A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds ... View More

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Immunogen image

Immunogen Image c-Cbl Polyclonal Antibody

Product Details

A302-337A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

Immunoprecipitation (IP)

2-5 µg/mg lysate

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 575 and 625 of human Casitas B-lineage lymphoma proto-oncogene.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cbl- c is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed). Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Through interactions with proteins containing SRC homology-2 (SH2) and SH3 domains, CBL proteins modulate downstream cell signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cas-Br-M (murine) ecotropic retroviral transforming sequence; Casitas B-lineage lymphoma proto-oncogene; Cbl proto-oncogene, E3 ubiquitin protein ligase; CBL-3; CBLC; E3 ubiquitin-protein ligase CBL; fragile site, folic acid type, rare, fra(11)(q23.3); oncogene CBL2; Proto-oncogene c-Cbl; RING finger protein 55; RING-type E3 ubiquitin transferase CBL; RNF57; Signal transduction protein CBL

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Gene Aliases: C-CBL; CBL; CBL2; FRA11B; NSLL; RNF55

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UniProt ID: (Human) P22681

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Entrez Gene ID: (Human) 867

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Function(s)

phosphotyrosine binding transcription factor activity, sequence-specific DNA binding ubiquitin-protein transferase activity signal transducer activity calcium ion binding protein binding zinc ion binding ligase activity SH3 domain binding protein kinase binding ephrin receptor binding ubiquitin protein ligase activity cadherin binding involved in cell-cell adhesion

Process(es)

regulation of transcription, DNA-templated cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway fibroblast growth factor receptor signaling pathway positive regulation of phosphatidylinositol 3-kinase signaling protein ubiquitination negative regulation of epidermal growth factor receptor signaling pathway protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process positive regulation of epidermal growth factor receptor signaling pathway positive regulation of receptor-mediated endocytosis cell-cell adhesion

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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c-Cbl Polyclonal Antibody

c-Cbl Antibody (A302-337A) in WB

Product Details

A302-337A

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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