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  • Primary Antibodies ›
  • Beta III Tubulin Antibodies

Invitrogen

beta-3 Tubulin Monoclonal Antibody (2G10)

Advanced Verification
10 Published Figures
31 References
View all (51) Beta III Tubulin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite beta-3 Tubulin Monoclonal Antibody (2G10)

  • Antibody Testing Data (15)
  • Published Figures (10)
  • Advanced Verification (3)
beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
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beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
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beta-3 Tubulin Antibody (MA1-118X) in ICC/IF

Immunofluorescence analysis of beta III tubulin (green) in the ectoderm derived from human ES cells. Embryoid bodies (EBs) were generated from the H9 embryonic stem cell line (WiCell Research Institute, WA09) using Gibco® KnockOut™ Serum Replacement. After four days in suspension culture, EBs were plated on Geltrex™-coated tissue culture-treated polystyrene and continuously cultured for 21 days. EB cultures... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
beta-3 Tubulin Antibody in Immunohistochemistry (Frozen) (IHC (F))
beta-3 Tubulin Antibody in Immunohistochemistry (Frozen) (IHC (F))
beta-3 Tubulin Antibody in Immunohistochemistry (Frozen) (IHC (F))
beta-3 Tubulin Antibody in Western Blot (WB)
beta-3 Tubulin Antibody in Western Blot (WB)
beta-3 Tubulin Antibody in Western Blot (WB)
beta-3 Tubulin Antibody in Flow Cytometry (Flow)
beta-3 Tubulin Antibody in Immunoprecipitation (IP)
beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)
beta-3 Tubulin Antibody in Immunohistochemistry (IHC)
beta-3 Tubulin Antibody in Immunohistochemistry (IHC)
beta-3 Tubulin Antibody in Immunohistochemistry (IHC)
beta-3 Tubulin Antibody in Immunohistochemistry (IHC)
beta-3 Tubulin Antibody in Western Blot (WB)
beta-3 Tubulin Antibody
beta-3 Tubulin Antibody
beta-3 Tubulin Antibody
beta-3 Tubulin Monoclonal Antibody (2G10)

Product Details

MA1-118X

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 4 publications 4 publications

Immunohistochemistry (IHC)

Assay-dependent
View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200
View 16 publications 16 publications

Flow Cytometry (Flow)

1:50
-

Immunoprecipitation (IP)

5 µg
-
Product Specifications

Species Reactivity

Bovine, Guinea pig, Hamster, Human, Mouse, Pig, Rabbit, Rat

Published species

Amphibian, Human, Mouse, Rabbit, Rat, Zebrafish

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2G10

Immunogen

A synthetic peptide corresponding to amino acids 436-450 from rat neuronal specific beta-3 tubulin.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536830

Product Specific Information

MA1-118 was successfully used to detect E18 Sparague Dawley primary cortical neurons.

MA1-118 can be used for immunofluorescence analysis of beta III tubulin in the ectoderm derived from human embryonic stem cells.

Western blot analysis of MA1-118 detects an ~50 kDa protein in neuronal-type cells. In bovine, a unknown band at ~32 kDa is also detected. MA1-118 shows specificity to beta-3 Tubulin and is non-reactive to lysates from non-neuronal cell types (e.g. HeLa cell lysate).

Target Information

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: beta 3 tubulin; beta tubulin 3; betaIII-tubulin; class III beta-tubulin; MC1R; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB; tubulin beta; Tubulin beta-3; Tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4; tubulin, beta, 4-like

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Gene Aliases: 3200002H15Rik; beta-4; BOS_17166; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; I79_000194; M(beta)3; M(beta)6; TUBB3; TUBB4

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UniProt ID: (Bovine) Q2T9S0, (Human) Q13509, (Mouse) Q9ERD7, (Rat) Q4QRB4

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Entrez Gene ID: (Rabbit) 100341581, (Bovine) 768070, (Human) 10381, (Mouse) 22152, (Rat) 246118, (Chinese hamster) 100760055

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Function(s)
GTPase activity structural constituent of cytoskeleton GTP binding protein binding peptide binding nucleotide binding
Process(es)
microtubule-based process mitotic nuclear division axon guidance neuron differentiation protein polymerization metabolic process cytoskeleton organization
It has to be done as per old AB suggested Products section.
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