Invitrogen

alpha Dystroglycan Polyclonal Antibody

Name: alpha Dystroglycan Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-28179

View all (7) alpha Dystroglycan antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (8)

alpha Dystroglycan Antibody in Immunocytochemistry (ICC/IF)

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alpha Dystroglycan Antibody (PA5-28179) in ICC/IF

alpha Dystroglycan Polyclonal Antibody detects DAG1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DAG1 stained by alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) diluted at 1:500. Red: Tau, stained by Phospho-Tau (Ser262) Polyclonal Antibody [GT287]diluted at 1:500. Blue: Fluoroshield with... View More

alpha Dystroglycan Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Dystroglycan Antibody in Western Blot (WB)

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Product Details

PA5-28179

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Horse, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human DAG1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.28 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2545655

Product Specific Information

Recommended positive controls: U87-MG, U87-MG membrane fraction extract.

Predicted reactivity: Mouse (92%), Rat (92%), Dog (92%), Cat (95%), Rabbit (94%), Rhesus Monkey (98%), Bovine (93%).

Target Information

Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha-dystroglycan; beta-dystroglycan; Dystroglycan; Dystroglycan 1; dystroglycan 1 (dystrophin-associated glycoprotein 1); dystrophin associated glycoprotein 1; Dystrophin-associated glycoprotein 1

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Gene Aliases: 156DAG; A3a; AGRNR; D9Wsu13e; DAG; DAG1; DG; Dp427; Dp71; MDDGA9; MDDGC7; MDDGC9

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UniProt ID: (Human) Q14118, (Mouse) Q62165

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Entrez Gene ID: (Human) 1605, (Mouse) 13138, (Rat) 114489

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Function(s)

virus receptor activity dystroglycan binding actin binding calcium ion binding protein binding structural constituent of muscle tubulin binding vinculin binding protein complex binding SH2 domain binding laminin-1 binding alpha-actinin binding

Process(es)

morphogenesis of an epithelial sheet membrane protein ectodomain proteolysis NLS-bearing protein import into nucleus cytoskeletal anchoring at plasma membrane Schwann cell development viral process calcium-dependent cell-matrix adhesion modulation by virus of host morphology or physiology nerve maturation myelination in peripheral nervous system negative regulation of cell migration microtubule anchoring negative regulation of MAPK cascade negative regulation of protein kinase B signaling epithelial tube branching involved in lung morphogenesis branching involved in salivary gland morphogenesis commissural neuron axon guidance basement membrane organization protein O-linked glycosylation regulation of gastrulation regulation of epithelial to mesenchymal transition regulation of embryonic cell shape extracellular matrix organization response to peptide hormone viral entry into host cell positive regulation of basement membrane assembly involved in embryonic body morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Dystroglycan Polyclonal Antibody

alpha Dystroglycan Antibody (PA5-28179) in ICC/IF

Product Details

PA5-28179

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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