Invitrogen

ZO-2 Polyclonal Antibody

Name: ZO-2 Polyclonal Antibody
Brand: Invitrogen
SKU: 71-1400

Advanced Verification

82 Published Figures

127 References

View all (18) ZO-2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

ZO-2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 87

ZO-2 Antibody (71-1400) in ICC/IF

Immunofluorescent analysis of ZO2/TJP2 Antibody was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO2/TJP2 Antibody (Product # 71-1400) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at roo... View More

ZO-2 Antibody in Immunohistochemistry (IHC)

ZO-2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

ZO-2 Antibody in Immunocytochemistry, Immunoprecipitation (ICC/IF, IP)

ZO-2 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

ZO-2 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

ZO-2 Antibody in Western Blot, Immunohistochemistry, Immunoprecipitation (WB, IHC, IP)

ZO-2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

ZO-2 Antibody — FIGURE 1: The absence of ZO-2 altered the cytoarchitecture of epithelial cells. (A) ZO-2 KD cells are bigger than parental MDCK cells. MDCK cells were fixed and processed for immunofluorescence with antibodies against ZO-1 and ZO-2. (B) ZO-2 KD MDCK cells have a bigger diameter than parental cells. The diameter of cells was estimated by comparing in a flow cytometer the FSC signals with those of the reference microspheres. Results from three independent experiments. Statistical analysis done with two-way analysis of variance (ANOVA) followed by Bonferroni's multiple comparison test. *p < 0.05, ***p < 0.001. (C) The amount of membrane surface is bigger in ZO-2 KD than in parental MDCK cells. Membrane surface was estimated by measuring the electrical capacitance in a whole-cell clamp configuration. The membrane surface of 33 parental cells and 36 ZO-2 KD cells was evaluated. Statistical analysis was done with Student's t test, ****p < 0.0001. (D) Left, the FSC of light in a flow cytometer shows that three different clones of ZO-2 KD cells exhibit an increased cell size in comparison to parental cells. Right, the increase in cell size in ZO-2 KD cell clone IC5, evaluated by the FSC of light in a flow cytometer, was partially rescued by expressing a ZO-2 construct with altered shRNA-binding sites. (E) The amount of microvilli varies among cells of the parental MDCK clone (left), but in ZO-2 KD MDCK cells, microvilli density is significantly higher than in parental cells. Long mem {KD}

Product Details

71-1400

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 47 publications 47 publications

Immunohistochemistry (IHC)

Assay-dependent

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 3 publications 3 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

View 32 publications 32 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 12 publications 12 publications

ChIP assay (ChIP)

View 1 publication 1 publication

in situ PLA (PLA)

View 1 publication 1 publication

GST Pull Down (GPD)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Dog, Fish, Human, Mouse, Pig, Plant, Rabbit, Rat, Sheep, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the central portion of the ZO-2 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533976

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Friedreich ataxia region gene X104 (tight junction protein ZO-2); Tight junction protein 2; tight junction protein 2 (zona occludens 2); Tight junction protein ZO-2; Tight junction protein ZO-2 (Zonula occludens 2 protein) (Zona occludens 2 protein) (Tight junction protein 2); zona occludens 2; Zona occludens protein 2; Zonula occludens protein 2

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Gene Aliases: C9DUPq21.11; DFNA51; DUP9q21.11; PFIC4; TJP2; X104; ZO-2; ZO2

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UniProt ID: (Dog) Q95168, (Human) Q9UDY2, (Mouse) Q9Z0U1

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Entrez Gene ID: (Dog) 403854, (Human) 9414, (Mouse) 21873

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Function(s)

protein binding guanylate kinase activity protein C-terminus binding protein domain specific binding protein binding, bridging cadherin binding involved in cell-cell adhesion

Process(es)

hippo signaling GMP metabolic process GDP metabolic process intestinal absorption TNFSF11-mediated signaling pathway establishment of endothelial intestinal barrier regulation of membrane permeability cell-cell adhesion negative regulation of osteoclast development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZO-2 Polyclonal Antibody

ZO-2 Antibody (71-1400) in ICC/IF

Product Details

71-1400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

in situ PLA (PLA)

GST Pull Down (GPD)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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