NeoBiotechnologies

ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody (2F3.2)

Name: ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody (2F3.2)
Brand: NeoBiotechnologies
SKU: 7535-MSM1-P0

View all (96) Zap-70 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)

ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody (7535-MSM1-P0) in Flow

Flow Cytometric analysis of PFA-fixed Jurkat cells. ZAP70 Mouse Monoclonal Antibody (2F3.2) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody in Immunocytochemistry (ICC/IF)

ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody in SDS-PAGE (SDS-PAGE)

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Immunogen image

Immunogen Image ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody (2F3.2)

Product Details

7535-MSM1-P0

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL

Immunocytochemistry (ICC/IF)

1-2 µg/mL

Flow Cytometry (Flow)

1-2 µg/10^6 cells

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2F3.2

Immunogen

Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; kinase ZAP70; Selective T cell defect; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; ZA70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

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Gene Aliases: ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70; ZAP70

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UniProt ID: (Human) P43403

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Entrez Gene ID: (Human) 7535

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Function(s)

phosphotyrosine binding protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding

Process(es)

adaptive immune response macrophage activation involved in immune response neutrophil activation involved in immune response protein phosphorylation inflammatory response immune response transmembrane receptor protein tyrosine kinase signaling pathway peptidyl-tyrosine phosphorylation T cell differentiation positive regulation of cell adhesion mediated by integrin intracellular signal transduction peptidyl-tyrosine autophosphorylation T cell activation B cell activation positive regulation of mast cell degranulation beta selection positive thymic T cell selection negative thymic T cell selection innate immune response positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of alpha-beta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of calcium-mediated signaling T cell receptor signaling pathway B cell receptor signaling pathway T cell aggregation T cell migration regulation of platelet aggregation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody (2F3.2)

ZAP70 (Chronic Lymphocytic Leukemia Marker) Antibody (7535-MSM1-P0) in Flow

Product Details

7535-MSM1-P0

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

References (0)

Bioinformatics

Performance Guarantee

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