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OriGene

WNT3 Monoclonal Antibody (OTI3G5), TrueMAB™

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Datasheet
Protocols
Questions & Answers

Cite WNT3 Monoclonal Antibody (OTI3G5), TrueMAB™

  • Antibody Testing Data (4)
WNT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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WNT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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WNT3 Antibody (CF801892) in IHC (P)

Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801892) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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WNT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
WNT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
WNT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
WNT3 Antibody in Western Blot (WB)
WNT3 Monoclonal Antibody (OTI3G5), TrueMAB™

Product Details

CF801892

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI3G5

Immunogen

Human recombinant protein fragment corresponding to amino acids 128-355 of human WNT3 produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

WNT3, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC131950; MGC138321; MGC138323; Proto-oncogene Int-4 homolog; Proto-oncogene Wnt-3; wingless-type MMTV integration site family member 3; wingless-type MMTV integration site family, member 3; WNT-3 proto-oncogene protein

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Gene Aliases: INT4; TETAMS; WNT3

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UniProt ID: (Human) P56703

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Entrez Gene ID: (Human) 7473

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Function(s)
frizzled binding protein binding protein domain specific binding receptor agonist activity
Process(es)
cell morphogenesis mesoderm formation gamete generation axon guidance anterior/posterior axis specification dorsal/ventral axis specification positive regulation of gene expression Wnt signaling pathway positive regulation of Wnt signaling pathway neuron differentiation embryonic forelimb morphogenesis embryonic hindlimb morphogenesis canonical Wnt signaling pathway involved in mesenchymal stem cell differentiation canonical Wnt signaling pathway involved in osteoblast differentiation cell fate commitment positive regulation of collateral sprouting in absence of injury negative regulation of axon extension involved in axon guidance Spemann organizer formation at the anterior end of the primitive streak canonical Wnt signaling pathway limb bud formation head morphogenesis mammary gland epithelium development cellular response to retinoic acid
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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