Invitrogen

WNT1 Polyclonal Antibody, PeproTech®

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Datasheet
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Questions & Answers
WNT1 Polyclonal Antibody, PeproTech®

Product Details

500-P250-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human Wnt-1
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930060

Product Specific Information

AA Sequence of recombinant protein: ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Wnt-1. Anti-Human Wnt-1-specific antibody was purified by affinity chromatography employing an immobilized Human Wnt-1 matrix.

Sandwich ELISA: To detect Human Wnt-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Wnt-1 (500-P250Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Wnt-1.

Western Blot: To detect Human Wnt-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Wnt-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Proto-oncogene Int-1 homolog; Proto-oncogene Wnt-1; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)

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Gene Aliases: BMND16; INT1; OI15; WNT1

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UniProt ID: (Human) P04628

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Entrez Gene ID: (Human) 7471

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Function(s)
frizzled binding cytokine activity morphogen activity protein domain specific binding transcription regulatory region DNA binding receptor agonist activity
Process(es)
embryonic axis specification branching involved in ureteric bud morphogenesis positive regulation of protein phosphorylation cell-cell signaling myoblast fusion positive regulation of cell proliferation response to wounding positive regulation of lamellipodium assembly negative regulation of cell-substrate adhesion Wnt signaling pathway spinal cord association neuron differentiation diencephalon development central nervous system morphogenesis cerebellum formation forebrain anterior/posterior pattern specification midbrain-hindbrain boundary maturation during brain development negative regulation of cell-cell adhesion negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway ubiquitin-dependent SMAD protein catabolic process midbrain development organ regeneration T cell differentiation in thymus cell proliferation in midbrain inner ear morphogenesis signal transduction in response to DNA damage positive regulation of insulin-like growth factor receptor signaling pathway canonical Wnt signaling pathway involved in negative regulation of apoptotic process cell fate commitment negative regulation of fat cell differentiation positive regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation neuron fate determination neuron development positive regulation of sequence-specific DNA binding transcription factor activity Spemann organizer formation canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway bone development positive regulation of dermatome development hepatocyte differentiation cellular response to peptide hormone stimulus hematopoietic stem cell proliferation positive regulation of canonical Wnt signaling pathway negative regulation of cell aging beta-catenin destruction complex disassembly Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation canonical Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation embryonic brain development negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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