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  • Primary Antibodies ›
  • VPS35 Antibodies

Invitrogen

VPS35 Monoclonal Antibody (5A9), PerCP

View all (29) VPS35 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite VPS35 Monoclonal Antibody (5A9), PerCP

  • Antibody Testing Data (7)
VPS35 Antibody in Immunocytochemistry (ICC/IF)
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VPS35 Antibody in Immunocytochemistry (ICC/IF)
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VPS35 Antibody (MA5-45941) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using A549 WT, VPS35 KO cells. Samples were incubated with VPS35 monoclonal antibody (Product # MA5-45941), followed by Donkey Anti-Mouse AlexaFluor 594. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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VPS35 Antibody in Immunocytochemistry (ICC/IF)
VPS35 Antibody in Western Blot (WB)
VPS35 Antibody in Western Blot (WB)
VPS35 Antibody in Immunoprecipitation (IP)
VPS35 Antibody in Immunoprecipitation (IP)
VPS35 Antibody in Immunoprecipitation (IP)
VPS35 Antibody in Immunoprecipitation (IP)
VPS35 Monoclonal Antibody (5A9), PerCP

Product Details

MA5-45941

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:200
-

Immunoprecipitation (IP)

1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5A9

Immunogen

Full length recombinant human VSP35
View immunogen

Conjugate

PerCP PerCP PerCP
  • APC 
  • FITC
  • Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932395

Product Specific Information

A 1:1,000 dilution of MA5-45941 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.|Detects approximately 92 kDa.

Target Information

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp434E1211; DKFZp434P1672; FLJ10752; FLJ13588; FLJ20388; hVPS35; Maternal embryonic message 3; Maternal-embryonic 3; vacuolar protein sorting 35 homolog; Vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35

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Gene Aliases: AI647796; MEM3; PARK17; TCCCTA00141; VPS35

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UniProt ID: (Human) Q96QK1, (Mouse) Q9EQH3

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Entrez Gene ID: (Human) 55737, (Rat) 25479, (Mouse) 65114

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Function(s)
protein binding protein transporter activity
Process(es)
intracellular protein transport Wnt signaling pathway regulation of macroautophagy regulation of protein stability retrograde transport, endosome to Golgi transcytosis positive regulation of Wnt protein secretion positive regulation of canonical Wnt signaling pathway negative regulation of late endosome to lysosome transport retrograde transport, endosome to plasma membrane vacuolar protein processing transport protein transport
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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