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  • Primary Antibodies ›
  • VPS33B Antibodies

Invitrogen

VPS33B Polyclonal Antibody

View all (11) VPS33B antibodies

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Datasheet
Protocols
Questions & Answers

Cite VPS33B Polyclonal Antibody

  • Antibody Testing Data (1)
VPS33B Antibody in Western Blot (WB)
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VPS33B Antibody in Western Blot (WB)
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VPS33B Antibody (PA5-97158) in WB

Western blot analysis of VPS33B in extracts of various cell lines. Samples were incubated with VPS33B Polyclonal antibody (Product # PA5-97158) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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VPS33B Antibody in Western Blot (WB)
VPS33B Polyclonal Antibody

Product Details

PA5-97158

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 348-617 of human VPS33B (NP_0611383)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.4 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2808960

Product Specific Information

Immunogen sequence: CESIMKKKTK QDFQELIKTE HALLEGFNIR ESTSYIEEHI DRQVSPIESL RLMCLLSITE NGLIPKDYRS LKTQYLQSYG PEHLLTFSNL RRAGLLTEQA PGDTLTAVES KVSKLVTDKA AGKITDAFSS LAKRSNFRAI SKKLNLIPRV DGEYDLKVPR DMAYVFGGAY VPLSCRIIEQ VLERRSWQGL DEVVRLLNCS DFAFTDMTKE DKASSESLRL ILVVFLGGCT FSEISALRFL GREKGYRFIF LTTAVTNSAR LMEAMSEVKA; Positive Samples: A-549, MCF7, DU145; Cellular Location: Cytoplasmic side, Cytoplasmic vesicle, Early endosome, Late endosome membrane, Lysosome membrane, Peripheral membrane protein, Recycling endosome, clathrin-coated vesicle

Target Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ14848; hVPS33B; vacuolar protein sorting 33 homolog B; vacuolar protein sorting 33-like protein B; Vacuolar protein sorting-associated protein 33B

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Gene Aliases: VPS33B

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UniProt ID: (Human) Q9H267, (Mouse) P59016

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Entrez Gene ID: (Human) 26276, (Mouse) 233405

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Function(s)
protein binding
Process(es)
transport vesicle docking involved in exocytosis protein transport vesicle-mediated transport melanosome localization lysosome localization membrane fusion platelet alpha granule organization endosome organization endosome to lysosome transport megakaryocyte development regulation of platelet aggregation autophagosome maturation
It has to be done as per old AB suggested Products section.
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