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  • Primary Antibodies ›
  • VCAM-1 (CD106) Antibodies

Invitrogen

VCAM-1 Polyclonal Antibody

Advanced Verification
3 Published Figures
3 References
View all (95) VCAM-1 (CD106) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite VCAM-1 Polyclonal Antibody

  • Antibody Testing Data (4)
  • Published Figures (3)
  • Advanced Verification (1)
VCAM-1 Antibody in Western Blot (WB)
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VCAM-1 Antibody in Western Blot (WB)
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VCAM-1 Antibody (PA5-47029) in WB

Western blot analysis of VCAM-1 lysates of C2C12 mouse myoblast cell line and 3T3-L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM-1/CD106 at approximately 116 kDa (as indicated) using 1 µg/mL VCAM-1 Polyclonal Antibody (Product # PA5-47029) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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VCAM-1 Antibody in Western Blot (WB)
VCAM-1 Antibody in Western Blot (WB)
VCAM-1 Antibody in Flow Cytometry (Flow)
VCAM-1 Antibody in Flow Cytometry (Flow)
VCAM-1 Antibody in Immunohistochemistry (IHC)
VCAM-1 Antibody in Immunohistochemistry (IHC)
VCAM-1 Antibody in Western Blot (WB)
VCAM-1 Antibody
VCAM-1 Polyclonal Antibody

Product Details

PA5-47029

Applications
Tested Dilution
Publications

Western Blot (WB)

0.25 µg/mL
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL
-

Flow Cytometry (Flow)

0.25 µg/10^6 cells
-
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VCAM-1/CD106 Phe25-Glu698
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609027

Product Specific Information

In direct ELISA and Western blots, approximately 5% cross-reactivity with recombinant human VCAM-1 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp779G2333; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; vascular cell adhesion protein 1

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Gene Aliases: CD106; Vcam-1

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Entrez Gene ID: (Mouse) 22329

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Function(s)
integrin binding primary amine oxidase activity cell adhesion molecule binding
Process(es)
cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion heart development amine metabolic process viral process single organismal cell-cell adhesion positive regulation of T cell proliferation leukocyte tethering or rolling oxidation-reduction process cell chemotaxis embryonic placenta morphogenesis chorio-allantoic fusion visceral serous pericardium development cellular response to glucose stimulus
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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