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  • Primary Antibodies ›
  • UNC84B Antibodies

Invitrogen

UNC84B Polyclonal Antibody

Advanced Verification
1 Reference
View all (8) UNC84B antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite UNC84B Polyclonal Antibody

  • Antibody Testing Data (9)
  • Advanced Verification (2)
UNC84B Antibody in Immunocytochemistry (ICC/IF)
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UNC84B Antibody in Immunocytochemistry (ICC/IF)
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UNC84B Antibody (PA5-51539) in ICC/IF

Immunofluorescence analysis of UNC84B was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with UNC84B Rabbit Polyclonal Antibody (Product # PA5-51539) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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UNC84B Antibody in Immunocytochemistry (ICC/IF)
UNC84B Antibody in Immunocytochemistry (ICC/IF)
UNC84B Antibody in Immunohistochemistry (IHC)
UNC84B Antibody in Immunohistochemistry (IHC)
UNC84B Antibody in Immunohistochemistry (IHC)
UNC84B Antibody in Immunohistochemistry (IHC)
UNC84B Antibody in Western Blot (WB)
UNC84B Antibody in Western Blot (WB)
UNC84B Antibody in Western Blot (WB)
UNC84B Antibody
UNC84B Antibody
UNC84B Polyclonal Antibody

Product Details

PA5-51539

Applications
Tested Dilution
Publications

Western Blot (WB)

0.04-0.4 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000
-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human UNC84B. Recombinant protein control fragment (Product #RP-91354).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2649276

Product Specific Information

Immunogen sequence: LKSEWQSMTQ ESFQESSVKE LRRLEDQLAG LQQELAALAL KQSSVAEEVG LLPQQIQAVR DDVESQFPAW ISQFLARGGG GRVGLLQREE MQAQLRELES KILTHVAEMQ GKSAREAAAS LSLTLQKEGV IGVTEEQVHH IVKQALQRYS E

Highest antigen sequence identity to the following orthologs: Mouse - 78%, Rat - 80%.

Target Information

UNC84B may form a physical interaction between the nuclear envelope and the centrosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: nuclear envelope protein; Protein unc-84 homolog B; Rab5-interacting protein; Rab5IP; Sad1 unc-84 domain protein 2; Sad1/unc-84 protein-like 2; SUN domain-containing protein 2; unc-84 homolog B

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Gene Aliases: FRIGG; KIAA0668; RAB5IP; SUN2; UNC84B

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UniProt ID: (Human) Q9UH99

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Entrez Gene ID: (Human) 25777

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Function(s)
protein binding lamin binding microtubule binding identical protein binding
Process(es)
nuclear envelope organization mitotic spindle organization nuclear migration positive regulation of cell migration nuclear migration along microfilament centrosome localization cytoskeletal anchoring at nuclear membrane nuclear matrix anchoring at nuclear membrane
It has to be done as per old AB suggested Products section.
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