Invitrogen

TRIM21 Polyclonal Antibody

Name: TRIM21 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-110517

Advanced Verification

View all (23) TRIM21 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (11)
Advanced Verification (2)

TRIM21 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

TRIM21 Antibody (PA5-110517) in ICC/IF

Immunofluorescence analysis of TRIM21 in NIH/3T3 cells. Samples were incubated with TRIM21 Polyclonal antibody (Product # PA5-110517) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

TRIM21 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Immunogen image

Immunogen Image TRIM21 Polyclonal Antibody

Product Details

PA5-110517

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

ELISA (ELISA)

1 µg/mL

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 86-334 of human TRIM21/SS-A (NP_0031322)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2855928

Product Specific Information

Immunogen sequence: GTQGERCAVH GERLHLFCEK DGKALCWVCA QSRKHRDHAM VPLEEAAQEY QEKLQVALGE LRRKQELAEK LEVEIAIKRA DWKKTVETQK SRIHAEFVQQ KNFLVEEEQR QLQELEKDER EQLRILGEKE AKLAQQSQAL QELISELDRR CHSSALELLQ EVIIVLERSE SWNLKDLDIT SPELRSVCHV PGLKKMLRTC AVHITLDPDT ANPWLILSED RRQVRLGDTQ QSIPGNEERF DSYPMVLGA

Target Information

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; 52kD Ro/SSA autoantigen; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; Ro(SS-A); Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SS-A; tripartite motif protein 21; tripartite motif-containing 21; Tripartite motif-containing protein 21

View more View less

Gene Aliases: RNF81; Ro/SSA; RO52; SSA; SSA1; TRIM21

View more View less

UniProt ID: (Human) P19474

View more View less

Entrez Gene ID: (Human) 6737, (Rat) 308901, (Mouse) 20821

View more View less

Function(s)

DNA binding RNA binding ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity identical protein binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Process(es)

protein polyubiquitination protein monoubiquitination protein ubiquitination protein destabilization negative regulation of NF-kappaB transcription factor activity negative regulation of viral transcription innate immune response positive regulation of cell cycle positive regulation of viral entry into host cell positive regulation of sequence-specific DNA binding transcription factor activity protein autoubiquitination protein trimerization negative regulation of protein deubiquitination negative regulation of viral release from host cell cell cycle positive regulation of autophagy regulation of type I interferon production response to interferon-gamma interferon-gamma-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

TRIM21 Polyclonal Antibody

TRIM21 Antibody (PA5-110517) in ICC/IF

Product Details

PA5-110517

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!