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  • TOP2A Antibodies

Invitrogen

TOP2A Monoclonal Antibody (6C12G12)

View all (93) TOP2A antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite TOP2A Monoclonal Antibody (6C12G12)

  • Antibody Testing Data (6)
TOP2A Antibody in Immunocytochemistry (ICC/IF)
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TOP2A Antibody in Immunocytochemistry (ICC/IF)
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TOP2A Antibody (MA5-38648) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of TOP2A in HeLa cells using TOP2A Monoclonal Antibody (Product # MA5-38648) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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TOP2A Antibody in Immunocytochemistry (ICC/IF)
TOP2A Antibody in Immunocytochemistry (ICC/IF)
TOP2A Antibody in Western Blot (WB)
TOP2A Antibody in Western Blot (WB)
TOP2A Antibody in Western Blot (WB)
TOP2A Antibody in Flow Cytometry (Flow)
TOP2A Monoclonal Antibody (6C12G12)

Product Details

MA5-38648

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Flow Cytometry (Flow)

1:200-1:400
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

6C12G12

Immunogen

Purified recombinant fragment of human TOP2A (aa: 1100-1530) expressed in E. Coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898560

Product Specific Information

This antibody has been tested in indirect ELISA.

Target Information

Topo II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2 and M-phases in transformed and developmentally regulated normal cellsTopo II alpha is also implicated in drug resistance of tumor cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; OTTHUMP00000207108; P11388-1; topoisomerase (DNA) 2 alpha; topoisomerase (DNA) II alpha 170kDa; topoisomerase ii alpha

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Gene Aliases: TOP2; TOP2A; TP2A

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UniProt ID: (Human) P11388

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Entrez Gene ID: (Human) 7153, (Rat) 360243

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Function(s)
magnesium ion binding DNA binding chromatin binding DNA topoisomerase type II (ATP-hydrolyzing) activity protein kinase C binding protein binding ATP binding protein C-terminus binding DNA-dependent ATPase activity drug binding DNA binding, bending enzyme binding protein homodimerization activity histone deacetylase binding ubiquitin binding poly(A) RNA binding protein heterodimerization activity DNA topoisomerase activity sequence-specific DNA binding structure-specific DNA binding
Process(es)
resolution of meiotic recombination intermediates sister chromatid segregation hematopoietic progenitor cell differentiation DNA topological change DNA ligation DNA unwinding involved in DNA replication mitotic recombination cellular response to DNA damage stimulus chromosome segregation protein sumoylation apoptotic chromosome condensation embryonic cleavage regulation of circadian rhythm positive regulation of apoptotic process mitotic DNA integrity checkpoint positive regulation of viral genome replication positive regulation of single stranded viral RNA replication via double stranded DNA intermediate positive regulation of transcription from RNA polymerase II promoter rhythmic process brain development aging cerebellar Purkinje cell differentiation cerebellar granule cell differentiation chromosome condensation response to drug response to parathyroid hormone
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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