Invitrogen

TOP1 Recombinant Rabbit Monoclonal Antibody (SC69-03)

Name: TOP1 Recombinant Rabbit Monoclonal Antibody (SC69-03)
Brand: Invitrogen
SKU: MA5-32228

Advanced Verification

1 Published Figure

1 Reference

View all (25) Topoisomerase I antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

TOP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

TOP1 Antibody (MA5-32228) in ICC/IF

Immunofluorescence analysis of TOP1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TOP1 Recombinant Rabbit Monoclonal Antibody (SC69-03) (Product # MA5-32228) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluo... View More

TOP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

TOP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with TOP1 siRNA and decrease in signal intensity was observed in western blot application using TOP1 Recombinant Rabbit Monoclonal Antibody (SC69-03) (Product # MA5-32228). {KD}

Immunogen image

Immunogen Image TOP1 Recombinant Rabbit Monoclonal Antibody (SC69-03)

Product Details

MA5-32228

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:1,000

Immunocytochemistry (ICC/IF)

1:50-1:500

Flow Cytometry (Flow)

1:50-1:100

ChIP assay (ChIP)

2.5 µg/10^6 cells

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SC69-03

Immunogen

Synthetic peptide within Human TOP1 aa 1-49

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2809514

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets.

Science advances

Roberts JA,Varma VR,An Y,Varma S,Candia J,Fantoni G,Tiwari V,Anerillas C,Williamson A,Saito A,Loeffler T,Schilcher I,Moaddel R,Khadeer M,Lovett J,Tanaka T,Pletnikova O,Troncoso JC,Bennett DA,Albert MS,Yu K,Niu M,Haroutunian V,Zhang B,Peng J,Croteau DL,Resnick SM,Gorospe M,Bohr VA,Ferrucci L,Thambisetty M

MA5-32228 was used in Western Blot to characterize and validate the precise mechanisms mediating APOE ε4-associated risk of Alzheimer’s disease (AD), and show that drugs targeting three of these proteins—STAT3, YES1, and FYN—rescue distinct molecular phenotypes relevant to AD pathogenesis.

Fri Nov 12 00:00:00 UTC 2021

A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets.

Science advances

Fri Nov 12 00:00:00 UTC 2021

A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets.

Science advances

Fri Nov 12 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: DNA topoisomerase 1; DNA topoisomerase I; RP3-511B24.2; type I DNA topoisomerase

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Gene Aliases: AI467334; D130064I21Rik; Top-1; TOP1; TOPI

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UniProt ID: (Human) P11387, (Mouse) Q04750

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Entrez Gene ID: (Human) 7150, (Mouse) 21969

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Function(s)

core promoter sequence-specific DNA binding DNA binding chromatin binding DNA topoisomerase type I activity DNA topoisomerase type II (ATP-hydrolyzing) activity protein binding poly(A) RNA binding DNA topoisomerase activity isomerase activity chromatin DNA binding protein complex binding

Process(es)

DNA replication DNA topological change chromatin remodeling chromosome segregation circadian rhythm programmed cell death viral process phosphorylation protein sumoylation circadian regulation of gene expression embryonic cleavage response to drug rRNA transcription rhythmic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TOP1 Recombinant Rabbit Monoclonal Antibody (SC69-03)

TOP1 Antibody (MA5-32228) in ICC/IF

Product Details

MA5-32228

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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