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  • Primary Antibodies ›
  • Topoisomerase I Antibodies

Invitrogen

TOP1 Polyclonal Antibody

View all (25) Topoisomerase I antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite TOP1 Polyclonal Antibody

  • Antibody Testing Data (3)
TOP1 Antibody in Immunocytochemistry (ICC/IF)
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TOP1 Antibody in Immunocytochemistry (ICC/IF)
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TOP1 Antibody (PA5-80174) in ICC/IF

Immunocytochemistry analysis of Topoisomerase I using anti-Topoisomerase I antibody (Product # PA5-80174) . Topoisomerase I was detected in a section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Topoisomerase I antibody (Product # PA5-80174) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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TOP1 Antibody in Immunocytochemistry (ICC/IF)
TOP1 Antibody in Western Blot (WB)
TOP1 Antibody in Western Blot (WB)
TOP1 Polyclonal Antibody

Product Details

PA5-80174

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived human Topoisomerase I recombinant protein (Position: E526-F765).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2747288

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human HEL whole cell, human Daudi whole cell, human MOLT-4 whole cell, human MCF-7 whole cell, rat brain tissue, mouse brain tissue. ICC/IF: Hela cell.

Target Information

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA topoisomerase 1; DNA topoisomerase I; RP3-511B24.2; type I DNA topoisomerase

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Gene Aliases: Ab2-086; AI467334; D130064I21Rik; Top-1; TOP1; TOPI

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UniProt ID: (Human) P11387, (Rat) Q9WUL0, (Mouse) Q04750

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Entrez Gene ID: (Human) 7150, (Rat) 64550, (Mouse) 21969

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Function(s)
core promoter sequence-specific DNA binding DNA binding chromatin binding DNA topoisomerase type I activity DNA topoisomerase type II (ATP-hydrolyzing) activity protein binding poly(A) RNA binding chromatin DNA binding protein complex binding DNA topoisomerase activity isomerase activity
Process(es)
DNA replication DNA topological change chromatin remodeling chromosome segregation circadian rhythm programmed cell death viral process phosphorylation protein sumoylation circadian regulation of gene expression embryonic cleavage response to drug response to temperature stimulus rRNA transcription response to gamma radiation organ regeneration response to cAMP cellular response to luteinizing hormone stimulus rhythmic process
It has to be done as per old AB suggested Products section.
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