Invitrogen

TLR2 Monoclonal Antibody (T2.5), FITC

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
TLR2 Monoclonal Antibody (T2.5), FITC

Product Details

MA5-49111

Applications
Tested Dilution
Publications

Immunohistochemistry (Frozen) (IHC (F))

1:50
-

Immunocytochemistry (ICC/IF)

1:50
-

Flow Cytometry (Flow)

1:50
-

Immunoprecipitation (IP)

1:50
-

Functional Assay (Functional)

1:50
-

Inhibition Assays (IA)

1:50
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

T2.5

Immunogen

The monoclonal antibody T2.5 recognizes mouse Toll-like receptor 2 (TLR)
View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

100 µg/mL

Purification

Protein G

Storage buffer

PBS with 1% BSA

Contains

0.02% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3093971

Target Information

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD282; TLR-2; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4

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Gene Aliases: CD282; Ly105; TIL4; TLR2

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UniProt ID: (Human) O60603, (Mouse) Q9QUN7

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Entrez Gene ID: (Human) 7097, (Mouse) 24088

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Function(s)
lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding signaling pattern recognition receptor activity Toll-like receptor binding triacyl lipopeptide binding peptidoglycan binding protein heterodimerization activity diacyl lipopeptide binding lipoteichoic acid binding lipopeptide binding
Process(es)
microglial cell activation toll-like receptor signaling pathway response to molecule of bacterial origin response to molecule of fungal origin cytokine secretion involved in immune response immune system process positive regulation of leukocyte migration regulation of dendritic cell cytokine production cell surface pattern recognition receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway leukotriene metabolic process inflammatory response immune response signal transduction I-kappaB phosphorylation negative regulation of cell proliferation response to bacterium positive regulation of Wnt signaling pathway central nervous system myelin formation response to bacterial lipoprotein response to peptidoglycan interleukin-10 production negative regulation of interleukin-12 production negative regulation of interleukin-17 production positive regulation of chemokine production positive regulation of interferon-beta production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of toll-like receptor signaling pathway toll-like receptor 2 signaling pathway positive regulation of NF-kappaB import into nucleus detection of triacyl bacterial lipopeptide detection of diacyl bacterial lipopeptide positive regulation of tumor necrosis factor biosynthetic process chloramphenicol transport negative regulation of growth of symbiont in host innate immune response positive regulation of nitric oxide biosynthetic process positive regulation of transcription from RNA polymerase II promoter nitric oxide metabolic process positive regulation of oligodendrocyte differentiation regulation of cytokine secretion positive regulation of cytokine secretion positive regulation of inflammatory response defense response to Gram-positive bacterium positive regulation of NF-kappaB transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process induction by symbiont of defense-related host nitric oxide production positive regulation of macrophage cytokine production response to fatty acid cellular response to bacterial lipopeptide cellular response to lipoteichoic acid cellular response to peptidoglycan cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic process positive regulation of gene expression lipopolysaccharide-mediated signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway
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