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  • TIF1 gamma Antibodies

Invitrogen

TIF1 gamma Polyclonal Antibody

Advanced Verification
View all (18) TIF1 gamma antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite TIF1 gamma Polyclonal Antibody

  • Antibody Testing Data (8)
  • Advanced Verification (2)
TIF1 gamma Antibody in Immunocytochemistry (ICC/IF)
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TIF1 gamma Antibody in Immunocytochemistry (ICC/IF)
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TIF1 gamma Antibody (PA5-78496) in ICC/IF

Immunofluorescence analysis of TIF1 gamma was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TIF1 gamma Polyclonal Antibody (Product # PA5-78496) at 1:100 dilution in 0.1% BSA, incubated at 4 degree cel... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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TIF1 gamma Antibody in Immunocytochemistry (ICC/IF)
TIF1 gamma Antibody in Immunocytochemistry (ICC/IF)
TIF1 gamma Antibody in Immunocytochemistry (ICC/IF)
TIF1 gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P))
TIF1 gamma Antibody in Western Blot (WB)
TIF1 gamma Antibody in Western Blot (WB)
TIF1 gamma Antibody in Western Blot (WB)
TIF1 gamma Antibody in Western Blot (WB)
TIF1 gamma Antibody
TIF1 gamma Antibody

Product Details

PA5-78496

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of Human TIF1 gamma. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2736702

Product Specific Information

Positive Control: MCF-7, MDA-MB-231

Predicted Reactivity: Mouse (97%), Xenopus laevis (91%), Bovine (97%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TIF1 gamma is a member of the Transcriptional Intermediary Factor 1 family, which serve as coactivators and corepressors. Its structure is similar to TIF1 alpha and TIF1 beta, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain). TIF1 gamma functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; Protein Rfg7; RET-fused gene 7 protein; RING-type E3 ubiquitin transferase TRIM33; TIF 1-g; TIF1-gamma; Transcription intermediary factor 1-gamma; transcriptional intermediary factor 1 gamma; tripartite motif-containing 33; Tripartite motif-containing protein 33

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Gene Aliases: ECTO; KIAA1113; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA; TRIM33

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UniProt ID: (Human) Q9UPN9

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Entrez Gene ID: (Human) 51592

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Function(s)
DNA binding ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity co-SMAD binding R-SMAD binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated protein ubiquitination regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway negative regulation of transcription, DNA-templated
It has to be done as per old AB suggested Products section.
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