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Invitrogen
TCR H-Y (male antigen) Monoclonal Antibody (T3.70), FITC, eBioscience™
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-Flow-20170216130641.jpg?time=20250104 "TCR H-Y (male antigen) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 1
TCR H-Y (male antigen) Antibody (11-9930-82) in Flow
-Flow-20170216130641.jpg?time=20250104)
-Flow-20170216130641.jpg?time=20250104?time=20250104 "TCR H-Y (male antigen) Antibody (11-9930-82) in Flow")
Product Details
11-9930-82
Species Reactivity
Published species
Host/Isotype
Recommended Isotype Control
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Conjugate
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Product Specific Information
Description: The T3.70 monoclonal antibody reacts with the transgenic alpha betaTCR that recognizes the male antigen H-Y in the context of H-2D^b. A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4^+ and CD8^+ T cell differentiation.
Applications Reported: The T3.70 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The T3.70 antibody has been tested by flow cytometric analysis of CD11.3 line, which is derived from a thymic tumor of the H-Y TCR transgenic mice and expresses the transgenic H-Y TCR. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4+ and CD8+ T cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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