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  • TCEB2 Antibodies

Invitrogen

TCEB2 Polyclonal Antibody

View all (17) TCEB2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite TCEB2 Polyclonal Antibody

  • Antibody Testing Data (7)
TCEB2 Antibody in Immunocytochemistry (ICC/IF)
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TCEB2 Antibody in Immunocytochemistry (ICC/IF)
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TCEB2 Antibody (PA5-144068) in ICC/IF

Immunocytochemistry analysis of TCEB2 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in TCEB2 Polyclonal antibody (Product # PA5-144068) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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TCEB2 Antibody in Immunocytochemistry (ICC/IF)
TCEB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
TCEB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
TCEB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
TCEB2 Antibody in Western Blot (WB)
TCEB2 Antibody in Flow Cytometry (Flow)
TCEB2 Antibody in Flow Cytometry (Flow)
TCEB2 Polyclonal Antibody

Product Details

PA5-144068

Applications
Tested Dilution
Publications

Western Blot (WB)

0.25-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human TCEB2/Elongin-B/ELOB recombinant protein (Position: M1-Q118).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3075282

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human K562 whole cell, human Hela whole cell. IHC: mouse pancreas tissue, rat pancreas tissue, human papillary carcinoma of the left breast tissue. ICC/IF: MCF-7 cell. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 18 kDa, elongin B; EloB; Elongin 18 kDa subunit; elongin B; elongin, 18-kD subunit; Elongin-B; RNA polymerase II transcription factor SIII p18 subunit; RNA polymerase II transcription factor SIII subunit B; SIII p18; TCEB 2; transcription elongation factor B (SIII) polypeptide 2 (18kD, elongin B); transcription elongation factor B (SIII), polypeptide 2 (18 kDa, elongin B); transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B); Transcription elongation factor B polypeptide 2

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Gene Aliases: 0610040H15Rik; ELOB; SIII; TCEB2

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UniProt ID: (Human) Q15370, (Mouse) P62869, (Rat) P62870

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Entrez Gene ID: (Human) 6923, (Mouse) 67673, (Rat) 81807

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Function(s)
protein binding ubiquitin protein ligase binding transcription coactivator activity protein complex binding transcription factor activity, RNA polymerase II transcription factor binding ubiquitin-protein transferase activity
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated transcription elongation from RNA polymerase II promoter positive regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein ubiquitination protein complex assembly regulation of transcription from RNA polymerase II promoter in response to hypoxia
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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