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  • Primary Antibodies ›
  • TBX5 Antibodies

Invitrogen

TBX5 Polyclonal Antibody

4 Published Figures
14 References
View all (7) TBX5 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite TBX5 Polyclonal Antibody

  • Antibody Testing Data (1)
  • Published Figures (4)
TBX5 Antibody in Immunocytochemistry (ICC/IF)
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TBX5 Antibody in Immunocytochemistry (ICC/IF)
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TBX5 Antibody (42-6500) in ICC/IF

Immunofluorescence analysis of TBX5 Polyclonal Antibody was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TBX5 Rabbit Polyclonal Antibody (Product # 42-6500) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conju... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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TBX5 Antibody in Immunocytochemistry (ICC/IF)
TBX5 Antibody in Immunohistochemistry, Immunoprecipitation (IHC, IP)
TBX5 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
TBX5 Antibody in Western Blot, Immunoprecipitation (WB, IP)
TBX5 Antibody in ChIP Assay (ChIP)

Product Details

42-6500

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
View 4 publications 4 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL
View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-dependent
View 2 publications 2 publications

ChIP assay (ChIP)

-
View 1 publication 1 publication

Miscellaneous PubMed (MISC)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of the human, predicted dog, and predicted chimpanzee Tbx5 proteins, which differ from mouse and rat by one conservative amino acid change

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533533

Target Information

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: T-box protein 5; T-box transcription factor TBX5

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Gene Aliases: HOS; TBX5

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UniProt ID: (Human) Q99593, (Mouse) P70326

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Entrez Gene ID: (Human) 6910, (Mouse) 21388

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding sequence-specific DNA binding
Process(es)
morphogenesis of an epithelium bundle of His development atrioventricular valve morphogenesis endocardial cushion development cardiac left ventricle formation ventricular septum development transcription initiation from RNA polymerase II promoter cell-cell signaling pattern specification process heart development negative regulation of cell proliferation negative regulation of epithelial to mesenchymal transition lung development embryonic limb morphogenesis negative regulation of cell migration embryonic forelimb morphogenesis forelimb morphogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of cardioblast differentiation cardiac muscle cell differentiation pericardium development negative regulation of cardiac muscle cell proliferation positive regulation of cardiac muscle cell proliferation transdifferentiation atrial septum morphogenesis cell migration involved in coronary vasculogenesis positive regulation of secondary heart field cardioblast proliferation regulation of execution phase of apoptosis ventricular cardiac muscle tissue development atrial septum development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development
It has to be done as per old AB suggested Products section.
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