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  • Primary Antibodies ›
  • TBCK Antibodies

Invitrogen

TBCK Polyclonal Antibody

View all (6) TBCK antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite TBCK Polyclonal Antibody

  • Antibody Testing Data (4)
TBCK Antibody in Immunocytochemistry (ICC/IF)
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TBCK Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 4

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TBCK Antibody (PA5-34789) in ICC/IF

Immunocytochemistry analysis of TBCK in paraformaldehyde-fixed HeLa. Samples were incubated with TBCK polyclonal antibody (Product # PA5-34789) using a dilution of 1:200. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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TBCK Antibody in Immunocytochemistry (ICC/IF)
TBCK Antibody in Immunohistochemistry (Paraffin) (IHC (P))
TBCK Antibody in Western Blot (WB)
TBCK Antibody in Western Blot (WB)

Product Details

PA5-34789

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human TBCK. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2552141

Product Specific Information

Recommended positive controls: HepG2, Molt-4, NIH-3T3, JC, BCL-1.

Predicted reactivity: Mouse (100%), Rat (99%), Zebrafish (98%), Xenopus laevis (98%), Chicken (100%).

IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Involved in the modulation of mTOR signaling and expression of mTOR complex components. Involved in the regulation of cell proliferation and growth. Involved in the control of actin-cytoskeleton organization.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FERRY endosomal RAB5 effector complex subunit 1; FERRY endosomal RAB5A effector complex subunit 1; Fy-1; TBC domain-containing protein kinase-like protein; TBCK

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Gene Aliases: 1700120J03Rik; 9430001M19; A630047E20Rik; C030007I09Rik; FERRY1; HSPC302; IHPRF3; TBCK; TBCKL

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UniProt ID: (Human) Q8TEA7, (Mouse) Q8BM85

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Entrez Gene ID: (Human) 93627, (Mouse) 271981

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Function(s)
protein kinase activity GTPase activator activity ATP binding Rab GTPase binding
Process(es)
protein phosphorylation intracellular protein transport regulation of vesicle fusion activation of GTPase activity
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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