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  • SEMA3F Antibodies

Alomone Labs, LTD

Semaphorin-3F Polyclonal Antibody

View all (8) SEMA3F antibodies

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Datasheet
Protocols
Questions & Answers

Cite Semaphorin-3F Polyclonal Antibody

  • Antibody Testing Data (2)
Semaphorin-3F Antibody in Western Blot (WB)
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Semaphorin-3F Antibody in Western Blot (WB)
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Semaphorin-3F Antibody (ASR-056-200UL) in WB

Western blot analysis of rat brain membranes (lanes 1 and 5), rat lung membranes (lanes 2 and 6), mouse heart membranes (lanes 3 and 7) and rat dorsal root ganglion lysate (lanes 4 and 8): - 1-4. Anti-Semaphorin-3F Antibody (#ASR-056), (1:400). 5-8. Anti-Semaphorin-3F Antibody , preincubated with Semaphorin-3F Blocking Peptide (#BLP-SR056) (#BLP-SR056). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Semaphorin-3F Antibody in Western Blot (WB)
Semaphorin-3F Antibody in Western Blot (WB)
Semaphorin-3F Polyclonal Antibody

Product Details

ASR-056-200UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:400
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)RERSAEAPQSPAVYAR, corresponding to amino acid residues 280-295 of human Semaphorin-3F, Secreted
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed 7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F; Sema III/F; Sema IV; semaphorin 3F; semaphorin III/F; Semaphorin IV; Semaphorin-3F

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Gene Aliases: SEMA-IV; SEMA3F; SEMA4; SemaIV; SEMAK

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UniProt ID: (Human) Q13275, (Mouse) O88632

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Entrez Gene ID: (Human) 6405, (Mouse) 20350, (Rat) 315996

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Function(s)
semaphorin receptor binding neuropilin binding chemorepellent activity receptor activity
Process(es)
neural crest cell migration axon guidance facial nerve structural organization trigeminal nerve structural organization nerve development branchiomotor neuron axon guidance ventral trunk neural crest cell migration negative regulation of axon extension involved in axon guidance axon extension involved in axon guidance negative chemotaxis sympathetic ganglion development sympathetic neuron projection extension sympathetic neuron projection guidance neural crest cell migration involved in autonomic nervous system development semaphorin-plexin signaling pathway involved in neuron projection guidance semaphorin-plexin signaling pathway involved in axon guidance positive regulation of cell migration semaphorin-plexin signaling pathway
It has to be done as per old AB suggested Products section.
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