Invitrogen

SUMO1 Polyclonal Antibody

Name: SUMO1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-24560

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View all (37) SUMO1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)
Advanced Verification (1)

SUMO1 Antibody in Immunocytochemistry (ICC/IF)

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SUMO1 Antibody (PA5-24560) in ICC/IF

Immunofluorescence analysis of SUMO1 (Small ubiquitin-related modifier 1) was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SUMO1 Polyclonal Antibody (Product # PA5-24560) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor ... View More

SUMO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SUMO1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with SUMO1, SUMO2, SUMO3 siRNAs and decrease in signal intensity was observed only in SUMO1 transfected cells in ICC application using Anti-SUMO1 Polyclonal Antibody (Product # PA5-24560), confirming the specificity of the antibody to SUMO1. {KD}

Product Details

PA5-24560

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

1:10-1:50

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

human SUMO1 recombinant protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2542060

Target Information

SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment, via an isopeptide bond, to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 is involved, for instance, in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. SUMO1 may also regulate a network of genes involved in palate development. Mutations in the gene can result in non-syndromic orofacial cleft 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CG4494-PA; GAP modifying protein 1; GAP-modifying protein 1; GMP1; LD07775p; OTTHUMP00000202403; OTTHUMP00000202404; OTTHUMP00000202405; OTTHUMP00000202406; OTTHUMP00000202409; OTTHUMP00000202410; Sentrin; Sentrin-1; Small ubiquitin-related modifier 1; small ubiquitin-related modifier-1; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1; Smt3C; SUMO-1; Ubiquitin-homology domain protein PIC1; ubiquitin-like 1 (sentrin); Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1

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Gene Aliases: DAP1; GMP1; OFC10; OK/SW-cl.43; PIC1; SENP2; SMT3; SMT3C; SMT3H3; SUMO1; UBL1

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UniProt ID: (Human) P63165, (Rat) Q5I0H3

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Entrez Gene ID: (Human) 7341, (Rat) 301442

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Function(s)

protein binding transcription factor binding potassium channel regulator activity protein tag ubiquitin protein ligase binding ion channel binding poly(A) RNA binding SUMO transferase activity

Process(es)

DNA repair double-strand break repair via nonhomologous end joining viral process protein sumoylation PML body organization positive regulation of protein complex assembly positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of protein localization negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of action potential negative regulation of transcription, DNA-templated protein stabilization palate development regulation of interferon-gamma-mediated signaling pathway global genome nucleotide-excision repair regulation of cardiac muscle cell contraction protein localization to nuclear pore positive regulation of calcium-transporting ATPase activity negative regulation of delayed rectifier potassium channel activity regulation of transcription, DNA-templated regulation of protein stability regulation of calcium ion transmembrane transport

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SUMO1 Polyclonal Antibody

SUMO1 Antibody (PA5-24560) in ICC/IF

Product Details

PA5-24560

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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