Invitrogen

SR-BI Polyclonal Antibody

Name: SR-BI Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-16788

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2 Published Figures

2 References

View all (26) SR-BI antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SR-BI Antibody in Immunocytochemistry (ICC/IF)

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SR-BI Antibody (PA1-16788) in ICC/IF

Immunocytochemistry analysis of SR-BI in HeLa cells. Samples were incubated with SR-BI polyclonal antibody (Product # PA1-16788) using a dilution of 2 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:500. Cells were fixed and permeabilized for 10 minutes using -20°C MeOH. Alpha tubulin (DM1A) was used as a co-stain at a 1:1,000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

SR-BI Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

SR-BI Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of SCARB1 was observed in HeLa, Hep G2, PC3 in comparison to Jurkat and MOLT-4 using Anti-SR-BI Polyclonal Antibody (Product # PA1-16788) in Western Blot. {RE}

Immunogen image

Immunogen Image SR-BI Polyclonal Antibody

Product Details

PA1-16788

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

2.5-5 µg/mL

Immunocytochemistry (ICC/IF)

1:50-1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

1:10-1:1,000

Immunoprecipitation (IP)

1:10-1:500

Product Specifications

Species Reactivity

Hamster, Human, Mustelid, Non-human primate, Pig, Rabbit

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2269859

Product Specific Information

Suggested positive control: antigen standard for SCARB1 (transient overexpression lysate).

Target Information

High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. The SR-BI binds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues, and mediates selective cholesterol uptake by a distinct mechanism. In mice, it seems that SR-BI plays a key role in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD36; CD36 and LIMPII analogous 1; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1; CD36 antigen-like 1; CD36-related class B scavenger receptor haSR-BI; CLA-1; Collagen type I receptor, thrombospondin receptor-like 1; HaSR-BI; High density lipoprotein receptor SR-BI; scavenger receptor b1; Scavenger receptor class B member 1; scavenger receptor class B type I; scavenger receptor class B type III; scavenger receptor class B, member 1; SR-BI; SRB1

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Gene Aliases: CD36L1; CLA-1; CLA1; haSR-bI; HDLQTL6; SCARB1; SR-BI; SRB1

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UniProt ID: (Pig) Q8SQC1, (Human) Q8WTV0, (Chinese hamster) Q60417

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Entrez Gene ID: (Rabbit) 100009590, (Pig) 397018, (Human) 949, (Chinese hamster) 100736552

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Function(s)

lipopolysaccharide binding virus receptor activity phosphatidylserine binding lipopolysaccharide receptor activity transporter activity protein binding 1-phosphatidylinositol binding high-density lipoprotein particle binding low-density lipoprotein particle binding apolipoprotein binding apolipoprotein A-I binding protein homodimerization activity high-density lipoprotein particle receptor activity

Process(es)

endothelial cell proliferation androgen biosynthetic process cholesterol catabolic process receptor-mediated endocytosis positive regulation of endothelial cell migration positive regulation of triglyceride biosynthetic process positive regulation of cholesterol storage regulation of phosphatidylcholine catabolic process phospholipid transport lipopolysaccharide transport lipopolysaccharide-mediated signaling pathway detection of lipopolysaccharide cholesterol efflux high-density lipoprotein particle remodeling low-density lipoprotein particle clearance high-density lipoprotein particle clearance vitamin transmembrane transport wound healing lipoprotein metabolic process cholesterol homeostasis blood vessel endothelial cell migration recognition of apoptotic cell reverse cholesterol transport adhesion of symbiont to host viral entry into host cell regulation of phagocytosis intestinal absorption positive regulation of nitric-oxide synthase activity triglyceride homeostasis cholesterol import

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SR-BI Polyclonal Antibody

SR-BI Antibody (PA1-16788) in ICC/IF

Product Details

PA1-16788

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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