Invitrogen

SP1 Monoclonal Antibody (3C4C3)

Name: SP1 Monoclonal Antibody (3C4C3)
Brand: Invitrogen
SKU: MA5-49142

View all (41) SP1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (10)

SP1 Antibody in Immunocytochemistry (ICC/IF)

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SP1 Antibody (MA5-49142) in ICC/IF

Immunocytochemistry analysis of SP1 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in SP1 Monoclonal antibody (Product # MA5-49142) using a dilution of 5 μg/mL. DyLight®550 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the... View More

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image SP1 Monoclonal Antibody (3C4C3)

Product Details

MA5-49142

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-0.5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3C4C3

Immunogen

E. coli-derived human SP1 recombinant protein (Position: Q384-A603).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_3074800

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human Caco-2 whole cell, human Jurkat whole cell. IHC: human colorectal adenocarcinoma tissue, human glioma tissue, human laryngeal squamous cell carcinoma tissue, human lung cancer tissue, human ovarian cancer tissue, human thyroid cancer tissue, human tonsil tissue. ICC/IF: Hela cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HGNC:11205; specificity protein 1; Transcription factor Sp1

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Gene Aliases: SP1; TSFP1

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UniProt ID: (Human) P08047

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Entrez Gene ID: (Human) 6667

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter sequence-specific DNA binding RNA polymerase II repressing transcription factor binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein C-terminus binding transcription factor binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding HMG box domain binding

Process(es)

regulation of transcription, DNA-templated viral process snRNA transcription from RNA polymerase II promoter positive regulation by host of viral transcription positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process regulation of phenotypic switching by transcription from RNA polymerase II promoter positive regulation of hydrogen sulfide biosynthetic process

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SP1 Monoclonal Antibody (3C4C3)

SP1 Antibody (MA5-49142) in ICC/IF

Product Details

MA5-49142

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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