Invitrogen

SNAP25 Monoclonal Antibody (SP12)

Name: SNAP25 Monoclonal Antibody (SP12)
Brand: Invitrogen
SKU: MA5-17609

Advanced Verification

2 Published Figures

3 References

View all (55) SNAP25 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SNAP25 Antibody in Immunocytochemistry (ICC/IF)

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SNAP25 Antibody (MA5-17609) in ICC/IF

Immunofluorescence analysis of Synaptosomal-associated protein 25 was performed using 70% confluent log phase PC-12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SNAP25 Monoclonal Antibody (SP12) (Product # MA5-17609) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody... View More

Product Image Gallery

Immunogen image

Immunogen Image SNAP25 Monoclonal Antibody (SP12)

Product Details

MA5-17609

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:2,000

Flow Cytometry (Flow)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

SP12

Immunogen

Synaptic preparation of post mortem human brain

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2538999

Product Specific Information

MA5-17609 targets SNAP-25 in ELISA, FACS, and WB applications and shows reactivity with Human samples.

The MA5-17609 immunogen is human Synaptobrevin 1 (aa 1-118) and Synaptobrevin 2(aa 33-96).

Target Information

SNAP25 is a t-SNARE involved in the molecular regulation of neurotransmitter release. It may play a role in the synaptic function of specific neuronal systems. SNAP25 associates with proteins involved in vesicle docking and membrane fusion. It also regulates plasma membrane recycling through its interaction with CENPF. Mutations in the gene can results in myasthenic syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ALMS1; blind drunk; CG17676; CG17884; CG40452; Dmel CG40452; Dmel_CG40452; FLJ23079; HGNC:11132; resistance to inhibitors of cholinesterase 4 homolog; SNAP 25; SNAP-25; snp25; SUP; Super protein; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; synaptosomal-associated protein, 25 kDa; synaptosomal-associated protein, 25kDa; synaptosome associated protein 25kDa

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Gene Aliases: bA416N4.2; Bdr; CMS18; dJ1068F16.2; GENA70; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SNAP-25a; SNAP-25B; SNAP25; sp; SUP

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UniProt ID: (Human) P60880, (Rat) P60881, (Mouse) P60879

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Entrez Gene ID: (Human) 6616, (Rat) 25012, (Mouse) 20614

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Function(s)

SNAP receptor activity protein binding syntaxin-1 binding calcium-dependent protein binding SNARE binding voltage-gated potassium channel activity myosin binding protein domain specific binding protein N-terminus binding ion channel binding

Process(es)

exocytosis neurotransmitter secretion axonogenesis long-term memory regulation of neuron projection development synaptic vesicle priming endosomal transport growth hormone secretion sleep synaptic vesicle fusion to presynaptic active zone membrane positive regulation of insulin secretion positive regulation of hormone secretion calcium ion-regulated exocytosis of neurotransmitter regulation of synapse assembly long-term synaptic potentiation regulation of establishment of protein localization potassium ion transmembrane transport neurotransmitter uptake chemical synaptic transmission locomotory behavior associative learning glutamate secretion synaptic vesicle exocytosis synaptic vesicle docking regulation of insulin secretion regulation of ion transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SNAP25 Monoclonal Antibody (SP12)

SNAP25 Antibody (MA5-17609) in ICC/IF

Product Details

MA5-17609

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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