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  • SMAD2 Antibodies

Invitrogen

SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4)

Advanced Verification
5 Published Figures
6 References
View all (66) SMAD2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4)

  • Antibody Testing Data (8)
  • Published Figures (5)
  • Advanced Verification (4)
SMAD2 Antibody in Immunocytochemistry (ICC/IF)
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SMAD2 Antibody in Immunocytochemistry (ICC/IF)
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SMAD2 Antibody (700048) in ICC/IF

Immunofluorescence analysis of SMAD2 was done on 70% confluent log phase TGF- beta treated HeLa cells (serum starved for 16 hours followed by treatment with 20 ng/mL TGF-beta for 1 hour). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SMAD2 Recombinant Rabbit Monoclonal Antibody (Product # 700048) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alex... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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SMAD2 Antibody in Immunocytochemistry (ICC/IF)
SMAD2 Antibody in Immunocytochemistry (ICC/IF)
SMAD2 Antibody in Western Blot (WB)
SMAD2 Antibody in Western Blot (WB)
SMAD2 Antibody in Western Blot (WB)
SMAD2 Antibody in Western Blot (WB)
SMAD2 Antibody in Flow Cytometry (Flow)
SMAD2 Antibody in ChIP Assay (ChIP)
SMAD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
SMAD2 Antibody in Western Blot (WB)
SMAD2 Antibody in Western Blot (WB)
SMAD2 Antibody in Western Blot (WB)
SMAD2 Antibody in ChIP Assay (ChIP)
SMAD2 Antibody
SMAD2 Antibody
SMAD2 Antibody
SMAD2 Antibody
SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4)

Product Details

700048

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-1 µg/mL
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-

ELISA (ELISA)

1-5 µg/mL
-

ChIP assay (ChIP)

3 µg
View 1 publication 1 publication

Miscellaneous PubMed (MISC)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

31H15L4

Immunogen

A peptide corresponding to amino acids 81-107 of Q15796.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532277

Product Specific Information

This antibody is predicted to react with bovine, carp, chicken, goldfish, mouse, opossum, orangutan, rat, Xenopus and zebrafish based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: hMAD-2; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2Deltaexon3

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Gene Aliases: hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; SMAD2

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UniProt ID: (Human) Q15796

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Entrez Gene ID: (Human) 4087

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transforming growth factor beta receptor binding protein binding transcription factor binding phosphatase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity ubiquitin protein ligase binding activating transcription factor binding type I transforming growth factor beta receptor binding enhancer binding protein homodimerization activity SMAD binding metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding R-SMAD binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ureteric bud development in utero embryonic development endoderm formation mesoderm formation transcription from RNA polymerase II promoter transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly zygotic specification of dorsal/ventral axis gastrulation negative regulation of cell proliferation response to glucose post-embryonic development anterior/posterior pattern specification positive regulation of epithelial to mesenchymal transition regulation of transforming growth factor beta receptor signaling pathway signal transduction involved in regulation of gene expression insulin secretion lung development negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway pancreas development primary miRNA processing activin receptor signaling pathway somatic stem cell population maintenance organ growth intracellular signal transduction nodal signaling pathway cell fate commitment negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm morphogenesis embryonic foregut morphogenesis embryonic cranial skeleton morphogenesis regulation of binding palate development pericardium development SMAD protein signal transduction response to cholesterol positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry
It has to be done as per old AB suggested Products section.
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