Invitrogen

SMAD1 Polyclonal Antibody

Name: SMAD1 Polyclonal Antibody
Brand: Invitrogen
SKU: 38-5400

Advanced Verification

31 Published Figures

23 References

View all (57) SMAD1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

SMAD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 36

SMAD1 Antibody (38-5400) in IHC (P)

Immunohistochemistry analysis of SMAD1 showing staining in the cytoplasm of paraffin-embedded mouse heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, was... View More

SMAD1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

SMAD1 Antibody in Immunohistochemistry (IHC)

SMAD1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

SMAD1 Antibody — Fig 1 TGF-beta inhibits BMP-induced transcriptional responses in an ALK5-dependent manner. (A, B, and E to G) MDA-MB-231 BRE cells and MDA-MB-231 CAGA cells were induced with the ligands indicated and assayed for luciferase and Renilla activities after 8 h. (C) C2C12 and HaCaT cells were transiently transfected with BRE-luciferase and TK- Renilla . After 24 h, the C2C12 cells were induced with the ligands shown and luciferase/ Renilla assays were performed. At 24 h after transfection, HaCaT cells were starved overnight in Opti-MEM before ligand induction. (D) MDA-MB-231 cells were transfected with ID2-luciferase and TK- Renilla for 24 h prior to ligand induction. In panel F, SB-431542 was added 15 min before the ligands shown, and in panel G, either dimethyl sulfoxide (DMSO) or SB-431542 was added 15, 30, or 60 min after ligand addition, as shown. All experiments were performed in triplicate. The data are presented as luciferase activity normalized to Renilla activity and are the mean and standard deviation from a single representative experiment. In panel E (right side), in parallel with the luciferase assay, MDA-MB-231 BRE cells were treated with ligands as indicated for 1 h. Whole-cell extracts were Western blotted using antibodies against PSmad1/5, PSmad2, and PSmad3, as well as total Smad1, -2, and -3. MCM7 is a loading control. The asterisk indicates a nonspecific band. {TM}

Product Details

38-5400

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 2 publications 2 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Affinity Purification (AP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

C. elegans, Human, Mouse, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of the human Smad1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533373

Target Information

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BSP-1; Dwarfin-A; Dwf-A; DWFC; hSMAD1; JV4-1; JV5-1; MAD homolog 1; MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; MADH5; mMad1; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; SMAD; SMAD 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1

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Gene Aliases: AI528653; BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; Mlp1; MusMLP; SMAD1

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UniProt ID: (Human) Q15797, (Mouse) P70340

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Entrez Gene ID: (Human) 4086, (Mouse) 17125

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding receptor signaling protein activity protein binding protein kinase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity identical protein binding protein homodimerization activity metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding DNA binding

Process(es)

MAPK cascade ureteric bud development mesodermal cell fate commitment osteoblast fate commitment transcription from RNA polymerase II promoter inflammatory response signal transduction transforming growth factor beta receptor signaling pathway SMAD protein complex assembly gamete generation negative regulation of cell proliferation embryonic pattern specification positive regulation of gene expression BMP signaling pathway midbrain development hindbrain development primary miRNA processing homeostatic process positive regulation of osteoblast differentiation positive regulation of transcription from RNA polymerase II promoter cartilage development cardiac muscle cell proliferation bone development SMAD protein signal transduction positive regulation of cartilage development positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein phosphorylation response to drug positive regulation of cell differentiation cellular response to organic cyclic compound cellular response to BMP stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SMAD1 Polyclonal Antibody

SMAD1 Antibody (38-5400) in IHC (P)

Product Details

38-5400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Affinity Purification (AP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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