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  • SLC5A5 Antibodies

Invitrogen

SLC5A5 Monoclonal Antibody (14F), PerCP

View all (16) SLC5A5 antibodies

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Cite SLC5A5 Monoclonal Antibody (14F), PerCP

  • Antibody Testing Data (5)
SLC5A5 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
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SLC5A5 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
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SLC5A5 Antibody (MA5-45491) in IHC (PFA)

Immunohistochemistry analysis using Trachea. Fixation involved 10% formalin solution for 12-24 hours at RT. Samples were incubated with Sodium Iodide Symporter monoclonal antibody (Product # MA5-45491) at 1:1,000 for 1 hour at RT, followed by HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain used was Mayer Hematoxylin (p... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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SLC5A5 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
SLC5A5 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
SLC5A5 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
SLC5A5 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
SLC5A5 Antibody in Western Blot (WB)
SLC5A5 Monoclonal Antibody (14F), PerCP

Product Details

MA5-45491

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1,000
-

Antibody Microarray (AM)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

14F

Immunogen

Mannose binding protein hNIS fusion (AA468-643)
View immunogen

Conjugate

PerCP PerCP PerCP
  • APC 
  • FITC
  • Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931945

Product Specific Information

1 µg/mL of MA5-45491 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at approximately 30 kDa, and approximately 15kDa.

Target Information

Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na(+)/I(-)-symporter; Na+/I- symporter; Natrium iodide transporter; Sodium-iodide symporter; Sodium/iodide cotransporter; solute carrier family 5 (sodium iodide symporter), member 5; solute carrier family 5 (sodium/iodide cotransporter), member 5; Solute carrier family 5 member 5; solute carrier family 5, member 5

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Gene Aliases: NIS; SLC5A5; TDH1

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UniProt ID: (Human) Q92911, (Rat) Q63008

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Entrez Gene ID: (Human) 6528, (Rat) 114613, (Mouse) 114479

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Function(s)
sodium:iodide symporter activity iodide transmembrane transporter activity symporter activity transporter activity
Process(es)
thyroid hormone generation sodium ion transport anion transport iodide transport sodium ion transmembrane transport cellular response to cAMP cellular response to gonadotropin stimulus anion transmembrane transport transport ion transport transmembrane transport
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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