Bethyl Laboratories

SIPA1 Polyclonal Antibody

Name: SIPA1 Polyclonal Antibody
Brand: Bethyl Laboratories
SKU: A302-028A

View all (13) SIPA1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

FIGURE: 1 / 1

SIPA1 Antibody (A302-028A) in WB

Detection of human and mouse SIPA1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SIPA1 antibody (Product # A302-028A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. SIPA1 was also immunoprecipitated by rabbit anti-SIPA1 antibody BL8146, which recognizes an upstream epitope. Detection: Chemiluminescence with exposur... View More

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Immunogen image

Immunogen Image SIPA1 Polyclonal Antibody

Product Details

A302-028A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

Immunoprecipitation (IP)

2-5 µg/mg lysate

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 992 to 1042 of Human signal-induced proliferation-associated protein 1.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

The product of this gene is a mitogen induced GTPase activating protein. It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GTPase-activating protein Spa-1; MGC102688; MGC17037; p130 SPA-1; signal-induced proliferation-associated gene 1; Signal-induced proliferation-associated protein 1; Sipa-1

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Gene Aliases: SIPA1; SPA1

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UniProt ID: (Human) Q96FS4

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Entrez Gene ID: (Human) 6494, (Mouse) 20469

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Function(s)

GTPase activator activity protein binding protein C-terminus binding

Process(es)

cytoskeleton organization negative regulation of cell adhesion signal transduction cell proliferation negative regulation of cell growth intracellular signal transduction cellular response to water deprivation positive regulation of GTPase activity negative regulation of cell cycle regulation of small GTPase mediated signal transduction regulation of cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SIPA1 Polyclonal Antibody

SIPA1 Antibody (A302-028A) in WB

Product Details

A302-028A

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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