Invitrogen

SHC Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers
SHC Polyclonal Antibody

Product Details

PA5-79995

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunohistochemistry (Frozen) (IHC (F))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

0.5-1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SHC (424-440aa DPSYVNVQNLDKARQAV).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2747110

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human A431 whole cell, human Hela whole cell, human HepG2 whole cell, human Jurkat whole cell, rat C6 whole cell, mouse thymus tissue, mouse RAW2467 whole cell, mouse NIH3T3 whole cell. IHC: Rat Brain Tissue, Human Lung Cancer Tissue IHC-F: Rat Intestine Tissue, Rat Brain Tissue. ICC/IF: A549 Cell.

Target Information

Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ26504; OTTHUMP00000035472; RP11-307C12.1; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC (Src homology 2 domain-containing) transforming protein 1; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; src homology 2 domain-containing transforming protein C1; Src homology 2 domain-containing-transforming protein C1

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Gene Aliases: p66; P66shc; SHC; SHC1; SHCA

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UniProt ID: (Human) P29353, (Rat) Q5M824, (Mouse) P98083

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Entrez Gene ID: (Human) 6464, (Rat) 85385, (Mouse) 20416

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Function(s)
phosphotyrosine binding transmembrane receptor protein tyrosine kinase adaptor activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding insulin receptor binding insulin-like growth factor receptor binding neurotrophin TRKA receptor binding protein binding phospholipid binding ephrin receptor binding protein phosphatase 2A binding protein kinase binding receptor tyrosine kinase binding protein complex binding phosphoprotein binding
Process(es)
MAPK cascade activation of MAPK activity angiogenesis response to hypoxia epidermal growth factor receptor signaling pathway heart development aging insulin receptor signaling pathway response to toxic substance response to organic cyclic compound single organismal cell-cell adhesion actin cytoskeleton organization neuron differentiation organ regeneration neuron projection development actin cytoskeleton reorganization response to insulin cellular response to insulin stimulus response to nicotine intracellular signal transduction regulation of growth regulation of cell proliferation response to hydrogen peroxide positive regulation of DNA replication positive regulation of vasoconstriction positive regulation of smooth muscle cell proliferation response to glucocorticoid regulation of epidermal growth factor-activated receptor activity Ras protein signal transduction positive regulation of cell proliferation viral process IRE1-mediated unfolded protein response Fc-epsilon receptor signaling pathway ERBB2 signaling pathway positive regulation of GTPase activity leukocyte migration cellular response to growth factor stimulus signal transduction
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