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Invitrogen

SF3B4 Monoclonal Antibody (3A1)

View all (12) SF3B4 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite SF3B4 Monoclonal Antibody (3A1)

  • Antibody Testing Data (2)
SF3B4 Antibody in Immunocytochemistry (ICC/IF)
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SF3B4 Antibody in Immunocytochemistry (ICC/IF)
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SF3B4 Antibody (MA5-47407) in ICC/IF

Immunofluorescent analysis of SF3B4 in HeLa cells. Samples were stained with SF3B4 monoclonal antibody (Product # MA5-47407) using a dilution of 1:1,000 (red). Costained with chicken pAb to vimentin dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The SF3B4 antibody reveals strong granularstaining of the nuclei, while the vimentin antibody specifically labels cytoplasmic intermediate filaments. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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SF3B4 Antibody in Immunocytochemistry (ICC/IF)
SF3B4 Antibody in Western Blot (WB)
SF3B4 Monoclonal Antibody (3A1)

Product Details

MA5-47407

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3A1

Immunogen

Full length recombinant human SF3B4 expressed in and purified from E. coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 50% glycerol

Contains

5mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942389

Target Information

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC10828; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SAP 49; SAP49MGC10828; SF3b50; spliceosomal protein; spliceosome-associated protein (U2 snRNP); Spliceosome-associated protein 49; Splicing factor 3B subunit 4; splicing factor 3b, subunit 4, 49kD; splicing factor 3b, subunit 4, 49kDa

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Gene Aliases: AFD1; Hsh49; SAP49; SF3B4; SF3b49; SF3b50

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UniProt ID: (Human) Q15427, (Mouse) Q8QZY9, (Rat) Q6AYL5

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Entrez Gene ID: (Human) 10262, (Mouse) 107701, (Rat) 295270

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Function(s)
nucleotide binding protein binding poly(A) RNA binding
Process(es)
mRNA processing RNA splicing positive regulation of mRNA splicing, via spliceosome RNA splicing, via transesterification reactions mRNA splicing, via spliceosome
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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