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  • Primary Antibodies ›
  • SETDB1 Antibodies

Invitrogen

SETDB1 Polyclonal Antibody

View all (17) SETDB1 antibodies

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Datasheet
Protocols
Questions & Answers

Cite SETDB1 Polyclonal Antibody

  • Antibody Testing Data (7)
SETDB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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SETDB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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SETDB1 Antibody (PA5-115483) in IHC (P)

Immunohistochemistry analysis of SETDB1 in mouse testis tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115483) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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SETDB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
SETDB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
SETDB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
SETDB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
SETDB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
SETDB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
SETDB1 Antibody in Western Blot (WB)
SETDB1 Polyclonal Antibody

Product Details

PA5-115483

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SETDB1(Accession Q15047), corresponding to amino acid residues D473-N523.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2900119

Product Specific Information

Antibody detects endogenous levels of total KMT1E / SETDB1.

Target Information

This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ERG-associated protein with a SET domain, ESET; ERG-associated protein with SET domain; ESET; H3-K9-HMTase 4; Histone H3-K9 methyltransferase 4; Histone-lysine N-methyltransferase SETDB1; histone-lysine N-methyltransferase, H3lysine-9 specific 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1; SET domain ERG-associated histone methyltransferase; SET domain, bifurcated 1; tudor domain containing 21

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Gene Aliases: AU022152; ESET; H3-K9-HMTase4; KG1T; KIAA0067; KMT1E; mKIAA0067; SETDB1; TDRD21

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UniProt ID: (Human) Q15047

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Entrez Gene ID: (Human) 9869, (Mouse) 84505, (Rat) 689883

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Function(s)
DNA binding chromatin binding protein binding zinc ion binding histone-lysine N-methyltransferase activity promoter-specific chromatin binding methyltransferase activity transferase activity metal ion binding molecular_function
Process(es)
negative regulation of transcription from RNA polymerase II promoter inner cell mass cell proliferation transcription, DNA-templated regulation of transcription, DNA-templated chromatin modification methylation histone lysine methylation histone H3-K9 trimethylation bone development response to vitamin response to ethanol Ras protein signal transduction positive regulation of methylation-dependent chromatin silencing
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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