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  • Primary Antibodies ›
  • SERCA2 ATPase Antibodies

Invitrogen

SERCA2 ATPase Polyclonal Antibody

1 Reference
View all (21) SERCA2 ATPase antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite SERCA2 ATPase Polyclonal Antibody

  • Antibody Testing Data (2)
SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
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SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
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SERCA2 ATPase Antibody (PA5-102354) in ICC/IF

Immunocytochemistry analysis of SERCA2 ATPase in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SERCA2 ATPase polyclonal antibody (Product # PA5-102354) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conj... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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SERCA2 ATPase Antibody in Immunocytochemistry (ICC/IF)
SERCA2 ATPase Antibody in Western Blot (WB)
SERCA2 ATPase Polyclonal Antibody

Product Details

PA5-102354

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ATP2A2(Accession P16615), corresponding to amino acid residues I638-E688.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2851761

Product Specific Information

Antibody detects endogenous levels of total ATP2A2.

Target Information

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATPase Ca++ transporting cardiac muscle slow twitch 2; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; ATPase, Ca++ transporting, slow twitch 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; cardiac Ca2+ ATPase; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarco(endo)plasmic reticulum Ca(2+)-dependent ATPase 2; sarco/endoplasmic reticulum Ca2+-ATPase 2; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA; SERCA ATPase; SERCA2; SR Ca(2+)-ATPase 2

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Gene Aliases: 9530097L16Rik; ATP2A2; ATP2B; D5Wsu150e; DAR; DD; mKIAA4195; SERCA2; Serca2a; SERCA2B; SercaII

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UniProt ID: (Human) P16615, (Mouse) O55143, (Rat) P11507

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Entrez Gene ID: (Human) 488, (Mouse) 11938, (Rat) 29693

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Function(s)
calcium-transporting ATPase activity calcium ion binding protein binding ATP binding protein C-terminus binding enzyme binding lutropin-choriogonadotropic hormone receptor binding S100 protein binding calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential nucleotide binding hydrolase activity metal ion binding
Process(es)
regulation of the force of heart contraction ER-nucleus signaling pathway organelle organization cell adhesion epidermis development positive regulation of heart rate regulation of cardiac muscle contraction by calcium ion signaling transition between fast and slow fiber endoplasmic reticulum calcium ion homeostasis positive regulation of endoplasmic reticulum calcium ion concentration T-tubule organization ion transmembrane transport cellular response to oxidative stress response to endoplasmic reticulum stress response to peptide hormone negative regulation of heart contraction relaxation of cardiac muscle sarcoplasmic reticulum calcium ion transport calcium ion transmembrane transport regulation of cardiac muscle cell membrane potential regulation of cardiac muscle cell action potential involved in regulation of contraction regulation of calcium ion-dependent exocytosis of neurotransmitter calcium ion transport from cytosol to endoplasmic reticulum regulation of cardiac conduction calcium ion import into sarcoplasmic reticulum transport ion transport calcium ion transport cellular calcium ion homeostasis regulation of muscle contraction metabolic process
It has to be done as per old AB suggested Products section.
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