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  • SEMA3B Antibodies

Invitrogen

SEMA3B Monoclonal Antibody (9C4F7)

View all (6) SEMA3B antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite SEMA3B Monoclonal Antibody (9C4F7)

  • Antibody Testing Data (3)
SEMA3B Antibody in Immunocytochemistry (ICC/IF)
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SEMA3B Antibody in Immunocytochemistry (ICC/IF)
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SEMA3B Antibody (MA5-49294) in ICC/IF

Immunocytochemistry analysis of SEMA3B in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in SEMA3B Monoclonal antibody (Product # MA5-49294) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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SEMA3B Antibody in Immunocytochemistry (ICC/IF)
SEMA3B Antibody in Western Blot (WB)
SEMA3B Antibody in Flow Cytometry (Flow)
SEMA3B Monoclonal Antibody (9C4F7)

Product Details

MA5-49294

Applications
Tested Dilution
Publications

Western Blot (WB)

0.25-0.5 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/10x^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

9C4F7

Immunogen

E.coli-derived human Semaphorin 3B/SEMA3B recombinant protein (Position: Y55-R624).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3074952

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human CACO-2 whole cell, human U87 whole cell, human K562 whole cell, rat kidney tissue, rat NRK whole cell, mouse kidney tissue, mouse ANA-1 whole cell. ICC/IF: Hela cell. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Seam V antibody; sema A; Sema A(V); sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B; Sema V; semaphorin A; Semaphorin-3B; semaphorin-A; Semaphorin-V

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Gene Aliases: LUCA-1; SemA; SEMA3B; SEMA5; SEMAA; semaV

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UniProt ID: (Human) Q13214

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Entrez Gene ID: (Human) 7869, (Mouse) 20347, (Rat) 363142

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Function(s)
semaphorin receptor binding neuropilin binding chemorepellent activity receptor activity
Process(es)
neural crest cell migration cell-cell signaling axon guidance positive regulation of cell migration negative regulation of axon extension involved in axon guidance negative chemotaxis semaphorin-plexin signaling pathway multicellular organism development nervous system development cell differentiation
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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