Invitrogen

S100B Monoclonal Antibody (SH-B4)

Name: S100B Monoclonal Antibody (SH-B4)
Brand: Invitrogen
SKU: MA1-25005

Advanced Verification

5 Published Figures

4 References

View all (97) S100B antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

S100B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

S100B Antibody (MA1-25005) in ICC/IF

Immunofluorescence analysis of Protein S100-B was performed using 70% confluent log phase SK-N-MC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with S100B Monoclonal Antibody (SH-B4) (Product # MA1-25005) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488... View More

S100B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

S100B Antibody in Immunohistochemistry (IHC)

Product Image Gallery

S100B Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-S100B Monoclonal Antibody (SH-B4) (Product # MA1-25005), shows expression of S100B was found higher in SK-N-MC cells as compared to SK-O-V3 cells. {RE}

Immunogen image

Immunogen Image S100B Monoclonal Antibody (SH-B4)

Product Details

MA1-25005

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Dog, Cat, Goat, Human, Mouse, Sheep, Pig, Rabbit, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

SH-B4

Immunogen

Bovine brain S-100b.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

Ascites

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_795382

Product Specific Information

Recommended positive controls: human tongue.

MA1-25005 has a predicted molecular weight of 10.5 kDa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Protein S100-B; S-100 calcium-binding protein beta subunit; S-100 calcium-binding protein, beta chain; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium binding protein beta (neural); S100 calcium-binding protein B; S100 calcium-binding protein beta (neural); S100 calcium-binding protein, beta (neural); S100 protein beta chain; S100 protein, beta polypeptide; S100 protein, beta polypeptide, neural

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Gene Aliases: AI850290; BOS_1447; Bpb; NEF; S100; S100-B; S100B; S100beta; S100P

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UniProt ID: (Human) P04271, (Bovine) P02638, (Rabbit) Q6YNR6, (Mouse) P50114, (Rat) P04631

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Entrez Gene ID: (Human) 6285, (Bovine) 525716, (Rabbit) 100009495, (Sheep) 101113190, (Dog) 491615, (Mouse) 20203, (Rat) 25742

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Function(s)

calcium ion binding protein binding zinc ion binding identical protein binding protein homodimerization activity S100 protein binding tau protein binding calcium-dependent protein binding RAGE receptor binding kinase inhibitor activity receptor binding metal ion binding

Process(es)

axonogenesis central nervous system development learning or memory memory cell proliferation positive regulation of cell proliferation regulation of cell shape positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response regulation of neuronal synaptic plasticity astrocyte differentiation response to glucocorticoid response to methylmercury long-term synaptic potentiation cellular response to hypoxia negative regulation of skeletal muscle cell differentiation regulation of translation phosphorylation negative regulation of phosphorylation positive regulation of complement activation astrocyte activation energy reserve metabolic process cellular calcium ion homeostasis regulation of cytokine biosynthetic process regulation of long-term neuronal synaptic plasticity positive regulation of synaptic transmission

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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S100B Monoclonal Antibody (SH-B4)

S100B Antibody (MA1-25005) in ICC/IF

Product Details

MA1-25005

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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