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  • S100A9 Antibodies

Invitrogen

S100A9 Polyclonal Antibody

Advanced Verification
View all (68) S100A9 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite S100A9 Polyclonal Antibody

  • Antibody Testing Data (8)
  • Advanced Verification (2)
S100A9 Antibody in Immunocytochemistry (ICC/IF)
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S100A9 Antibody in Immunocytochemistry (ICC/IF)
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S100A9 Antibody (PA5-19075) in ICC/IF

Immunocytochemistry analysis of S100A9 using S100A9 Polyclonal Antibody (Product # PA5-19075) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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S100A9 Antibody in Immunocytochemistry (ICC/IF)
S100A9 Antibody in Immunocytochemistry (ICC/IF)
S100A9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
S100A9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
S100A9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
S100A9 Antibody in Western Blot (WB)
S100A9 Antibody in Western Blot (WB)
S100A9 Antibody in Flow Cytometry (Flow)
S100A9 Antibody
S100A9 Antibody
S100A9 Polyclonal Antibody

Product Details

PA5-19075

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2.5 µg/mL
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

Flow Cytometry (Flow)

10 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (DTNADKQLSFEEF ) corresponding to the internal amino acids of S100A9 (aa 67-79).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10985454

Product Specific Information

This antibody is tested in Peptide ELISA: antibody detection limit dilution 8,000.

Target Information

S100A9 (MRP-14) is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BM-009; calgranulin B; Calgranulin-B; Calprotectin L1H subunit; DKFZp686B04128; Leukocyte L1 complex heavy chain; Macrophage; Migration inhibitory factor-related protein 14; Monocyte; MRP-14; MRP15; MRP16; Protein S100-A9; S100 A9; S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B)

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Gene Aliases: 60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100A9

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UniProt ID: (Human) P06702

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Entrez Gene ID: (Human) 6280

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Function(s)
signal transducer activity calcium ion binding protein binding microtubule binding zinc ion binding antioxidant activity Toll-like receptor 4 binding arachidonic acid binding RAGE receptor binding
Process(es)
cytokine production leukocyte migration involved in inflammatory response positive regulation of peptide secretion regulation of translation autophagy apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response signal transduction cell-cell signaling astrocyte development positive regulation of blood coagulation positive regulation of cell growth neutrophil chemotaxis actin cytoskeleton reorganization sequestering of zinc ion chemokine production peptidyl-cysteine S-trans-nitrosylation defense response to bacterium innate immune response regulation of integrin biosynthetic process positive regulation of inflammatory response defense response to fungus positive regulation of NF-kappaB transcription factor activity regulation of cytoskeleton organization neutrophil aggregation cellular oxidant detoxification positive regulation of intrinsic apoptotic signaling pathway
It has to be done as per old AB suggested Products section.
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