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  • RhoA/RhoB/RhoC Antibodies

Invitrogen

RhoA/RhoB/RhoC Recombinant Rabbit Monoclonal Antibody (SR38-00)

View all (3) RhoA/RhoB/RhoC antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite RhoA/RhoB/RhoC Recombinant Rabbit Monoclonal Antibody (SR38-00)

  • Antibody Testing Data (8)
RhoA/RhoB/RhoC Antibody in Immunocytochemistry (ICC/IF)
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RhoA/RhoB/RhoC Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 8

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RhoA/RhoB/RhoC Antibody (MA5-31993) in ICC/IF

Immunocytochemical analysis of RhoA/RhoB/RhoC in HepG2 cells using a RhoA/RhoB/RhoC Monoclonal antibody (Product # MA5-31993) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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RhoA/RhoB/RhoC Antibody in Immunocytochemistry (ICC/IF)
RhoA/RhoB/RhoC Antibody in Immunocytochemistry (ICC/IF)
RhoA/RhoB/RhoC Antibody in Immunocytochemistry (ICC/IF)
RhoA/RhoB/RhoC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RhoA/RhoB/RhoC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RhoA/RhoB/RhoC Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RhoA/RhoB/RhoC Antibody in Western Blot (WB)
RhoA/RhoB/RhoC Antibody in Flow Cytometry (Flow)
RhoA/RhoB/RhoC Recombinant Rabbit Monoclonal Antibody (SR38-00)

Product Details

MA5-31993

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:50-1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SR38-00

Immunogen

Recombinant protein within Human RhoC aa aa 1-193
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809287

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

RhoA is described as a small GTPase protein that regulates the actin cytoskeleton in the formation of stress fibers. RhoA is also included in the related proteins of the Ras superfamily, which involves proteins in the regulation and timing of cell division.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Aplysia ras-related homolog 12; Aplysia RAS-related homolog 6; aplysia ras-related homolog 9 (RhoC); aplysia ras-related homolog A; aplysia ras-related homolog A1; aplysia ras-related homolog A2; aplysia ras-related homolog B; h12; h6; h9; oncogene RHO H12; oncogene RHO H6; oncogene RHO H9; plysia ras-related homolog A2; Ras family member A; ras homolog 9 (RhoC); ras homolog A1; ras homolog A2; ras homolog B; ras homolog gene family, member A; ras homolog gene family, member A1; ras homolog gene family, member A2; ras homolog gene family, member AB; ras homolog gene family, member B; ras homolog gene family, member C; RAS-related homolog 9; Rho; Rho cDNA clone 12; Rho cDNA clone 6; Rho cDNA clone 9; Rho family GTPase; Rho-related GTP-binding protein RhoB; Rho-related GTP-binding protein RhoC; rhoB; rhoC GTPase; SIG-61; Silica-induced gene 61 protein; small GTP binding protein RhoA; small GTP binding protein RhoC; Transforming protein RhoA

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Gene Aliases: AA017882; AI324259; ARH12; ARH6; ARH9; ARHA; Arha1; Arha2; ARHB; ARHC; H9; MST081; MSTP081; RHO12; RHOA; RHOB; RHOC; RHOH12; RHOH6; RHOH9

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UniProt ID: (Human) P61586, (Human) P62745, (Human) P08134, (Rat) P61589, (Mouse) Q9QUI0, (Rat) P62747, (Mouse) P62746, (Mouse) Q62159

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Entrez Gene ID: (Human) 387, (Human) 388, (Human) 389, (Rat) 117273, (Mouse) 11848, (Rat) 64373, (Mouse) 11852, (Mouse) 11853, (Rat) 295342

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Function(s)
GTPase activity protein binding GTP binding myosin binding GDP binding signal transducer activity protein domain specific binding Rho GDP-dissociation inhibitor binding nucleotide binding
Process(es)
cytokinesis small GTPase mediated signal transduction positive regulation of I-kappaB kinase/NF-kappaB signaling apical junction assembly wound healing, spreading of cells regulation of small GTPase mediated signal transduction positive regulation of lipase activity skeletal muscle satellite cell migration signal transduction cell morphogenesis response to hypoxia regulation of transcription from RNA polymerase II promoter cytoskeleton organization cell adhesion cell-matrix adhesion skeletal muscle tissue development regulation of actin polymerization or depolymerization metabolic process response to mechanical stimulus response to glucose regulation of neuron projection development substantia nigra development cerebral cortex cell migration forebrain radial glial cell differentiation actin cytoskeleton organization cell differentiation positive regulation of cell growth regulation of cell migration positive regulation of cell migration androgen receptor signaling pathway positive regulation of actin filament polymerization stress-activated protein kinase signaling cascade positive regulation of cytokinesis negative regulation of intracellular steroid hormone receptor signaling pathway regulation of osteoblast proliferation cleavage furrow formation apolipoprotein A-I-mediated signaling pathway response to drug negative regulation of I-kappaB kinase/NF-kappaB signaling stress fiber assembly response to amino acid positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process ossification involved in bone maturation response to ethanol negative regulation of neuron differentiation positive regulation of neuron differentiation positive regulation of translation positive regulation of cell adhesion positive regulation of vasoconstriction positive regulation of smooth muscle contraction neuron projection morphogenesis regulation of dendrite development negative chemotaxis response to glucocorticoid positive regulation of stress fiber assembly regulation of calcium ion transport negative regulation of cell death trabecula morphogenesis cellular response to progesterone stimulus positive regulation of podosome assembly mitotic spindle assembly regulation of neural precursor cell proliferation cell cycle integrin-mediated signaling pathway Rho protein signal transduction GTP metabolic process cell division angiogenesis intracellular protein transport obsolete transformed cell apoptotic process endosome to lysosome transport positive regulation of apoptotic process positive regulation of angiogenesis negative regulation of cell cycle cellular response to hydrogen peroxide cellular response to ionizing radiation transforming growth factor beta receptor signaling pathway viral process cell migration platelet activation actin cytoskeleton reorganization regulation of actin cytoskeleton organization Roundabout signaling pathway positive regulation of NF-kappaB import into nucleus endothelial cell migration vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway phosphatidylinositol-mediated signaling negative regulation of axonogenesis positive regulation of axonogenesis Wnt signaling pathway, planar cell polarity pathway positive regulation of protein serine/threonine kinase activity negative regulation of cell migration involved in sprouting angiogenesis endothelial tube lumen extension mitotic cleavage furrow formation regulation of cell motility transport apoptotic process multicellular organism development protein transport positive regulation of endothelial cell migration negative regulation of cell migration endothelial tube morphogenesis
It has to be done as per old AB suggested Products section.
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