Disclaimer

Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

  • Isotype Controls ›
  • Rat IgG2a kappa Isotype Controls

Invitrogen

Rat IgG2a kappa Isotype Control (eBR2a), eBioscience™

2 Published Figures
View all Rat IgG2a kappa Isotype Controls

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Create Citation
Questions & Answers
Datasheet
Create Citation
Questions & Answers

Cite Rat IgG2a kappa Isotype Control (eBR2a), eBioscience™

  • Testing Data (14)
  • Published Figures (2)
Rat IgG2a kappa Isotype Control in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.
Rat IgG2a kappa Isotype Control in Immunocytochemistry (ICC/IF)
Group 53 Created with Sketch.

FIGURE: 1 / 16

{{ $ctrl.currentElement.advancedVerification.fullName }}
{{ $ctrl.currentElement.advancedVerification.text }}

Rat IgG2a kappa Isotype Control (14-4321-82) in ICC/IF

Immunofluorescence analysis of USP7 Monoclonal Antibody was performed using 60% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 1% Triton X-100 for 30 minutes at 37730C, and blocked with 10% Normal Goat Serum for 1 hour at room temperature. The cells were labeled with 1 µg/mL of USP7 Monoclonal Antibody (Product # 14-6907-8... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
Published figure supplied by benchsci-logo
PMID: {{$ctrl.currentElement.benchSciPubmedId}}
View Product

{{$ctrl.videoDesc}}

{{$ctrl.videoLongDesc}}

Rat IgG2a kappa Isotype Control in Immunocytochemistry (ICC/IF)
Rat IgG2a kappa Isotype Control in Immunocytochemistry (ICC/IF)
Rat IgG2a kappa Isotype Control in Immunocytochemistry (ICC/IF)
Rat IgG2a kappa Isotype Control in Immunohistochemistry (Paraffin) (IHC (P))
Rat IgG2a kappa Isotype Control in Immunohistochemistry (Paraffin) (IHC (P))
Rat IgG2a kappa Isotype Control in Immunohistochemistry (Paraffin) (IHC (P))
Rat IgG2a kappa Isotype Control in Immunohistochemistry (Paraffin) (IHC (P))
Rat IgG2a kappa Isotype Control in Immunohistochemistry (Paraffin) (IHC (P))
Rat IgG2a kappa Isotype Control in Immunohistochemistry (Frozen) (IHC (F))
Rat IgG2a kappa Isotype Control in Immunohistochemistry (Frozen) (IHC (F))
Rat IgG2a kappa Isotype Control in Immunohistochemistry (Frozen) (IHC (F))
Rat IgG2a kappa Isotype Control in Immunohistochemistry (Frozen) (IHC (F))
Rat IgG2a kappa Isotype Control in Immunohistochemistry (IHC)
Rat IgG2a kappa Isotype Control in Flow Cytometry (Flow)
Rat IgG2a kappa Isotype Control in Immunohistochemistry (Paraffin) (IHC (P))
Rat IgG2a kappa Isotype Control in Flow Cytometry (Flow)

Product Details

14-4321-82

Applications
Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

Flow Cytometry (Flow)

Assay-Dependent

Control (Ctrl)

Assay-Dependent
Product Specifications

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Isotype Control

Clone

eBR2a

Conjugate

Unconjugated Unconjugated Unconjugated
  • Alexa Fluor 488 
  • Alexa Fluor 532 
  • Alexa Fluor 561
  • Alexa Fluor 647 
  • Alexa Fluor 660 
  • Alexa Fluor 700 
  • APC 
  • APC-eFluor 780
  • Biotin 
  • Brilliant UV 395
  • Brilliant UV 496
  • Brilliant UV 563
  • Brilliant UV 615
  • Brilliant UV 661
  • Brilliant UV 737
  • Brilliant UV 805
  • Brilliant Violet 421
  • Brilliant Violet 480
  • Brilliant Violet 605
  • Brilliant Violet 650
  • Brilliant Violet 711
  • Brilliant Violet 786
  • eFluor 450
  • eFluor 506
  • eFluor 570
  • eFluor 615
  • eFluor 660
  • FITC
  • Functional Grade
  • Pacific Orange
  • PE 
  • PE-Cyanine5
  • PE-Cyanine5.5
  • PE-Cyanine7
  • PE-eFluor 610
  • PerCP-Cyanine5.5
  • PerCP-eFluor 710
  • Super Bright 436
  • Super Bright 600
  • Super Bright 645
  • Super Bright 702
  • Super Bright 780
  • Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_470105

Product Specific Information

Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.

Applications Reported: This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, flow cytometric analysis, and immunoglobulin ELISA.

Applications Tested: This rat IgG2a isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

View product citations for antibody 14-4321-82 on CiteAb

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product
It has to be done as per old AB suggested Products section.
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support

Your items have has been added!


Host server : magellan-search-5c796d7db4-nk9d7:80/100.66.128.142:80.
git-commit: d3b3aa98da0ae1b146c174fddb0766cfa6979daf
git-url: https://github.com/thermofisher/magellan-search
git-branch: release/2.36.2-2025.09.39-1.0