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  • Isotype Controls ›
  • Rat IgG1 kappa Isotype Controls

Invitrogen

Rat IgG1 kappa Isotype Control (eBRG1), eBioscience™

View all Rat IgG1 kappa Isotype Controls

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Cite Rat IgG1 kappa Isotype Control (eBRG1), eBioscience™

  • Testing Data (3)
Rat IgG1 kappa Isotype Control in Immunohistochemistry (Frozen) (IHC (F))
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Rat IgG1 kappa Isotype Control in Immunohistochemistry (Frozen) (IHC (F))
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Rat IgG1 kappa Isotype Control (14-4301-82) in IHC (F)

Immunohistochemistry of frozen mouse intestine stained with 2.5 µg/mL Rat IgG1 K Isotype Control Purified (left) or 2.5 µg/mL Anti-Mouse Lyve-1 Purified (right) followed by 5 µg/mL Anti-Rat IgG1 eFluor® 615. Nuclei are stained with DAPI. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Rat IgG1 kappa Isotype Control in Immunohistochemistry (Frozen) (IHC (F))
Rat IgG1 kappa Isotype Control in Immunohistochemistry (Frozen) (IHC (F))
Rat IgG1 kappa Isotype Control in Immunohistochemistry (Frozen) (IHC (F))

Product Details

14-4301-82

Applications
Tested Dilution

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

Flow Cytometry (Flow)

Assay-Dependent

Control (Ctrl)

Assay-Dependent
Product Specifications

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Isotype Control

Clone

eBRG1

Conjugate

Unconjugated Unconjugated Unconjugated
  • Alexa Fluor 488 
  • Alexa Fluor 532 
  • Alexa Fluor 561
  • Alexa Fluor 647 
  • Alexa Fluor 660 
  • Alexa Fluor 700 
  • APC 
  • APC-eFluor 780
  • Biotin 
  • Brilliant UV 395
  • Brilliant UV 563
  • Brilliant UV 615
  • Brilliant UV 661
  • Brilliant UV 737
  • Brilliant UV 805
  • Brilliant Violet 421
  • Brilliant Violet 480
  • Brilliant Violet 605
  • Brilliant Violet 650
  • Brilliant Violet 711
  • Brilliant Violet 786
  • eFluor 450
  • eFluor 506
  • eFluor 570
  • eFluor 615
  • eFluor 660
  • FITC
  • Functional Grade
  • Pacific Orange
  • PE 
  • PE-Cyanine5
  • PE-Cyanine7
  • PE-eFluor 610
  • PerCP-Cyanine5.5
  • PerCP-eFluor 710
  • Super Bright 436
  • Super Bright 600
  • Super Bright 645
  • Super Bright 702
  • Super Bright 780
  • Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_470102

Product Specific Information

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.

Applications Reported: This rat IgG1 isotype control has been reported for use in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and immunoglobulin ELISA.

Applications Tested: This rat IgG1 isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics. These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants. Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases. With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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