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  • Rab4 Antibodies

Invitrogen

Rab4 Polyclonal Antibody

Advanced Verification
5 Published Figures
10 References
View all (13) Rab4 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Rab4 Polyclonal Antibody

  • Antibody Testing Data (9)
  • Published Figures (5)
  • Advanced Verification (3)
Rab4 Antibody in Immunocytochemistry (ICC/IF)
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Rab4 Antibody in Immunocytochemistry (ICC/IF)
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Rab4 Antibody (PA3-912) in ICC/IF

Immunofluorescence analysis of RAB4A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RAB4A Rabbit polyclonal Antibody (Product # PA3-912) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Rab4 Antibody in Immunocytochemistry (ICC/IF)
Rab4 Antibody in Immunocytochemistry (ICC/IF)
Rab4 Antibody in Immunocytochemistry (ICC/IF)
Rab4 Antibody in Immunocytochemistry (ICC/IF)
Rab4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Rab4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Rab4 Antibody in Western Blot (WB)
Rab4 Antibody in Western Blot (WB)
Rab4 Antibody in Western Blot (WB)
Rab4 Antibody in Immunocytochemistry (ICC/IF)
Rab4 Antibody in Immunocytochemistry (ICC/IF)
Rab4 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
Rab4 Antibody in Western Blot (WB)
Rab4 Antibody in Western Blot (WB)
Rab4 Antibody
Rab4 Antibody
Rab4 Antibody

Product Details

PA3-912

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
View 4 publications 4 publications

Immunohistochemistry (IHC)

1:200
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:250
View 6 publications 6 publications
Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Published species

Bovine, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified, recombinant human Rab4 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2269382

Product Specific Information

PA3-912 detects Rab4 from human and mouse samples. This antibody does not detect other Rab isoforms (Rab1, Rab3, Rab5, or Rab6).

PA3-912 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~24 kDa band representing Rab4 from transfected HeLa cells.

The PA3-912 immunogen is purified recombinant human Rab4 protein.

Target Information

Protein transport. Probably involved in vesicular traffic.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HTLV-1 related endogenous sequence; Ras-related protein Rab-4A; Ras-related small GTP binding protein 4

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Gene Aliases: AI848268; HRES-1; HRES-1/RAB4; HRES1; RAB4; RAB4A

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UniProt ID: (Human) P20338, (Rat) P05714, (Mouse) P56371

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Entrez Gene ID: (Human) 5867, (Dog) 479210, (Rat) 25532, (Mouse) 19341

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Function(s)
ATPase activator activity GTPase activity protein binding GTP binding protein transporter activity GDP binding syntaxin binding ionotropic glutamate receptor binding ATPase binding nucleotide binding
Process(es)
protein transport antigen processing and presentation regulation of endocytosis Rab protein signal transduction positive regulation of ATPase activity intracellular protein transport small GTPase mediated signal transduction metabolic process transport
It has to be done as per old AB suggested Products section.
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