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Invitrogen
ROBO1 Polyclonal Antibody
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FIGURE: 1 / 5
ROBO1 Antibody (PA5-29917) in ICC/IF
Product Details
PA5-29917
Species Reactivity
Published species
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Conjugate
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Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, MDA-Mb-361, HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1).
Predicted reactivity: Mouse (100%), Rat (97%), Zebrafish (80%), Xenopus laevis (95%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Deleted in U twenty twenty; FLJ21882; H-Robo-1; HGNC:10249; MGC131599; MGC133277; roundabout 1; Roundabout homolog 1; roundabout, axon guidance receptor, homolog 1
Gene Aliases: AW494633; AW742721; DUTT1; Gm310; ROBO1; SAX3
UniProt ID: (Human) Q9Y6N7, (Mouse) O89026
Entrez Gene ID: (Human) 6091, (Chicken) 395292, (Mouse) 19876
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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