Invitrogen

RFP Recombinant Superclonal™ Antibody

Name: RFP Recombinant Superclonal\u2122 Antibody
Brand: Invitrogen
SKU: 710530

Advanced Verification

1 Reference

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Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Advanced Verification (2)

RFP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

RFP Antibody (710530) in ICC/IF

Immunofluorescent analysis of RFP in HEK293 cells using a RFP Recombinant Rabbit Superclonal™ Antibody (Product # 710530). Image A: HEK293 cells transduced by Tubulin-RFP. B: DAPI stained transduced cells. C: Merged images of an And B. D: CellLight Tubulin-RFP (Product # C10614) transduced HEK293 cells quenched by Red Fluorescent Protein Recombinant Rabbit Superclonal™ Antibody (Product # 710530). E: DAPI stained quenched cells. F: Merged images of D and E.

Product Image Gallery

RFP Antibody — Neutralization of Red Fluorescent Protein Activity by 710530: Fluorescence assay of red firefly luciferase using product 710530, showing the decreased fluorescence of red fluorescent protein in the presence of RFP antibody concentration. Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. See more information on neutralization verification of antibody selectivity. {Neu}

Product Details

710530

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-2 µg/mL

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Immunogen

Peptide corresponding to amino acids 32-45 of Red Fluorescent Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532732

Product Specific Information

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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