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  • RFC2 Antibodies

OriGene

RFC2 Monoclonal Antibody (OTI2B2), TrueMAB™

View all (31) RFC2 antibodies

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Datasheet
Protocols
Questions & Answers

Cite RFC2 Monoclonal Antibody (OTI2B2), TrueMAB™

  • Antibody Testing Data (13)
RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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FIGURE: 1 / 13

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RFC2 Antibody (CF505033) in IHC (P)

Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-RFC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505033) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RFC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RFC2 Antibody in Western Blot (WB)
RFC2 Antibody in Flow Cytometry (Flow)
RFC2 Monoclonal Antibody (OTI2B2), TrueMAB™

Product Details

CF505033

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-

Flow Cytometry (Flow)

1:100
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2B2

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-234 of human RFC2 produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A1 40 kDa subunit; Activator 1 40 kDa subunit; Activator 1 subunit 2; MGC3665; replication factor C (activator 1) 2, 40kDa; Replication factor C 40 kDa subunit; Replication factor C subunit 2; RF-C 40 kDa subunit

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Gene Aliases: RFC2; RFC40

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UniProt ID: (Human) P35250

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Entrez Gene ID: (Human) 5982

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Function(s)
DNA clamp loader activity protein binding ATP binding enzyme binding single-stranded DNA-dependent ATPase activity
Process(es)
telomere maintenance via recombination DNA replication transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling translesion synthesis nucleotide-excision repair, DNA incision error-prone translesion synthesis DNA damage response, detection of DNA damage error-free translesion synthesis positive regulation of DNA-directed DNA polymerase activity regulation of signal transduction by p53 class mediator
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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