Invitrogen

RAD50 Monoclonal Antibody (3D2G10)

Name: RAD50 Monoclonal Antibody (3D2G10)
Brand: Invitrogen
SKU: MA5-38619

View all (23) RAD50 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)

RAD50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

RAD50 Antibody (MA5-38619) in IHC (P)

Immunohistochemistry (Paraffin) analysis of RAD50 in paraffin-embedded esophageal cancer tissues using RAD50 Monoclonal Antibody (Product # MA5-38619) and DAB staining.

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Immunogen image

Immunogen Image RAD50 Monoclonal Antibody (3D2G10)

Product Details

MA5-38619

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3D2G10

Immunogen

Purified recombinant fragment of human RAD50 (aa: 228-359) expressed in E. Coli.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2898531

Product Specific Information

This antibody has been tested in indirect ELISA.

Target Information

Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA repair protein RAD50; hRAD50; hsRAD50; RAD50 2; RAD50 homolog, double strand break repair protein; RAD50 PEN; RAD50-2; RAD50-3

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Gene Aliases: hRad50; NBSLD; RAD50; RAD502

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UniProt ID: (Human) Q92878, (Rat) Q9JIL8

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Entrez Gene ID: (Human) 10111, (Rat) 64012

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Function(s)

single-stranded DNA endodeoxyribonuclease activity DNA binding double-stranded telomeric DNA binding ATP-dependent DNA helicase activity adenylate kinase activity protein binding ATP binding 3'-5' exonuclease activity protein binding, bridging single-stranded telomeric DNA binding metal ion binding G-quadruplex DNA binding ATPase activity

Process(es)

regulation of mitotic recombination telomere maintenance DNA catabolic process, endonucleolytic DNA repair double-strand break repair DNA recombination cellular response to DNA damage stimulus telomere maintenance via telomerase reciprocal meiotic recombination regulation of mitotic cell cycle positive regulation of protein autophosphorylation DNA duplex unwinding positive regulation of kinase activity negative regulation of viral entry into host cell chromosome organization protein heterooligomerization chromosome organization involved in meiotic cell cycle nucleic acid phosphodiester bond hydrolysis telomere maintenance via recombination double-strand break repair via homologous recombination DNA double-strand break processing DNA synthesis involved in DNA repair strand displacement DNA replication double-strand break repair via nonhomologous end joining viral process telomeric 3' overhang formation positive regulation of telomere maintenance nucleotide phosphorylation regulation of signal transduction by p53 class mediator negative regulation of telomere capping

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAD50 Monoclonal Antibody (3D2G10)

RAD50 Antibody (MA5-38619) in IHC (P)

Product Details

MA5-38619

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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