Invitrogen

RAD50 Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
RAD50 Polyclonal Antibody

Product Details

PA5-87368

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-270 of human Rad50 (NP_0057232)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.56 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804098

Product Specific Information

Positive Samples: NIH3T3, MCF-7, A-549, HT-29, Mouse lung; Cellular Location: Chromosome, Nucleus, telomere

Immunogen sequence: MSRIEKMSIL GVRSFGIEDK DKQIITFFSP LTILVGPNGA GKTTIIECLK YICTGDFPPG TKGNTFVHDP KVAQETDVRA QIRLQFRDVN GELIAVQRSM VCTQKSKKTE FKTLEGVITR TKHGEKVSLS SKCAEIDREM ISSLGVSKAV LNNVIFCHQE DSNWPLSEGK ALKQKFDEIF SATRYIKALE TLRQVRQTQG QKVKEYQMEL KYLKQYKEKA CEIRDQITSK EAQLTSSKEI VKSYENELDP LKNRLKEIEH NLSKIMKLDN

Target Information

Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA repair protein RAD50; hRAD50; hsRAD50; mRad50; RAD50 2; RAD50 homolog, double strand break repair protein; RAD50 PEN; RAD50-2; RAD50-3

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Gene Aliases: hRad50; Mrell; NBSLD; RAD50; RAD502; Rad50l

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UniProt ID: (Human) Q92878

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Entrez Gene ID: (Human) 10111, (Mouse) 19360

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Function(s)
single-stranded DNA endodeoxyribonuclease activity DNA binding double-stranded telomeric DNA binding ATP-dependent DNA helicase activity adenylate kinase activity protein binding ATP binding 3'-5' exonuclease activity protein binding, bridging single-stranded telomeric DNA binding metal ion binding G-quadruplex DNA binding nucleotide binding double-stranded DNA binding hydrolase activity ATPase activity
Process(es)
regulation of mitotic recombination telomere maintenance DNA repair double-strand break repair DNA recombination cellular response to DNA damage stimulus telomere maintenance via telomerase cell cycle regulation of mitotic cell cycle positive regulation of protein autophosphorylation DNA duplex unwinding positive regulation of kinase activity negative regulation of viral entry into host cell chromosome organization protein heterooligomerization meiotic cell cycle chromosome organization involved in meiotic cell cycle nucleic acid phosphodiester bond hydrolysis telomere maintenance via recombination double-strand break repair via homologous recombination DNA double-strand break processing DNA synthesis involved in DNA repair strand displacement DNA replication double-strand break repair via nonhomologous end joining reciprocal meiotic recombination viral process telomeric 3' overhang formation positive regulation of telomere maintenance nucleotide phosphorylation regulation of signal transduction by p53 class mediator negative regulation of telomere capping
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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