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  • Primary Antibodies ›
  • RACGAP1 Antibodies

OriGene

RACGAP1 Monoclonal Antibody (OTI4C5), TrueMAB™

View all (34) RACGAP1 antibodies

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Datasheet
Protocols
Questions & Answers

Cite RACGAP1 Monoclonal Antibody (OTI4C5), TrueMAB™

  • Antibody Testing Data (3)
RACGAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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RACGAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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RACGAP1 Antibody (CF811640) in IHC (P)

Immunohistochemical staining of paraffin-embedded human testicle tissue within the normal limits using anti-RACGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA811640)(1:150). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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RACGAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
RACGAP1 Antibody in Western Blot (WB)
RACGAP1 Antibody in Western Blot (WB)
RACGAP1 Monoclonal Antibody (OTI4C5), TrueMAB™

Product Details

CF811640

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI4C5

Immunogen

Human recombinant protein fragment corresponding to amino acids 123-238 of human RACGAP1 produced in E.coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

RAC, a small GTPase in the ras family, regulates the formation of membrane ruffles, lamellipodia and filopodia. It is involved in cytoskeletal actin organization and transformation. The p21 activated kinases (PAKs) are direct targets of active Rac and Cdc42 which can induce the assembly of polarized cytoskeletal structures when expressed in fibroblasts. RAC1 and RAC2 are also essential components of NADPH oxidase, the enzyme responsible for generating free radicals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AKT; CYK4; GAP; ID; Male germ cell RacGap; MGC99656; MgcRacGAP; PKB; PKB-ALPHA; PRKBA; protein CYK4 homolg; Protein CYK4 homolog; RAC; Rac GTPase-activating protein 1; RAC-ALPHA; racgap1

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Gene Aliases: AI227039; AI327394; Band25; CYK4; gtl11; GTPase; HsCYK-4; ID-GAP; KIAA1478; MGCRACGAP; mKIAA1478; RACGAP1

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UniProt ID: (Human) Q9H0H5, (Mouse) Q9WVM1

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Entrez Gene ID: (Human) 29127, (Mouse) 26934, (Rat) 315298

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Function(s)
GTPase activator activity protein binding phosphatidylinositol-3,4,5-trisphosphate binding microtubule binding protein kinase binding alpha-tubulin binding gamma-tubulin binding metal ion binding beta-tubulin binding lipid binding
Process(es)
mitotic cytokinesis actomyosin contractile ring assembly retrograde vesicle-mediated transport, Golgi to ER microtubule-based movement spermatogenesis neuroblast proliferation sulfate transport embryo development antigen processing and presentation of exogenous peptide antigen via MHC class II positive regulation of cytokinesis intracellular signal transduction positive regulation of GTPase activity regulation of small GTPase mediated signal transduction mitotic spindle midzone assembly regulation of attachment of spindle microtubules to kinetochore transport ion transport cell cycle signal transduction multicellular organism development cell differentiation cell division mitotic spindle assembly
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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