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  • RAC1/RAC2/RAC3 Antibodies

Invitrogen

RAC1/RAC2/RAC3 Polyclonal Antibody

1 Published Figure
1 Reference
View all (5) RAC1/RAC2/RAC3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite RAC1/RAC2/RAC3 Polyclonal Antibody

  • Antibody Testing Data (1)
  • Published Figures (1)
RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
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RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
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RAC1/RAC2/RAC3 Antibody (PA5-17519) in WB

Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), Jurkat (Lane 3), U-87 MG (Lane 4), 3T3-L1 (Lane 5) and NIH/3T3 (Lane 6). The blot was probed with Anti-RAC1/RAC2/RAC2 Polyclonal Antibody (Product # PA5-17519, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 20 kDa band corresponding to RAC1/RAC2/RAC3 was observed across the cell lin... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
RAC1/RAC2/RAC3 Polyclonal Antibody

Product Details

PA5-17519

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 1 publication 1 publication
Product Specifications

Species Reactivity

Bovine, Human, Mouse, Non-human primate, Rat, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues surrounding Thr138 of human Rac1
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

39 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986181

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other small GTPases.

Target Information

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cell migration-inducing gene 5 protein; EN-7 protein; GX; p21-Rac1; p21-Rac2; p21-Rac3; Protein EN-7; Ras-like protein TC25; RAS-related C3 botulinum substrate 2; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1); ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) L homeolog; Ras-related C3 botulinum toxin substrate 2; Ras-related C3 botulinum toxin substrate 3; ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3); Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2); rho family, small GTP binding protein Rac1; rho family, small GTP binding protein Rac3; Small G protein

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Gene Aliases: AI323801; AI452260; AL023026; BOS_19382; BOS_22937; BOS_5710; D5Ertd559e; EN-7; Gx; HSPC022; MIG5; p21-Rac1; p21-Rac2; Rac; Rac-1; RAC1; Rac1B; RAC2; RAC3; TC-25; TC25; XELAEV_18045321mg; Xrac

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UniProt ID: (Bovine) P62998, (Human) P63000, (Human) P15153, (Bovine) Q9TU25, (Human) P60763, (Mouse) P63001, (Rat) Q6RUV5, (Mouse) Q05144, (Mouse) P60764

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Entrez Gene ID: (Bovine) 281440, (Human) 5879, (Human) 5880, (Bovine) 327671, (Human) 5881, (Bovine) 619066, (Mouse) 19353, (Rat) 363875, (Mouse) 19354, (Rat) 366957, (Mouse) 170758, (Rat) 688319, (Xenopus) 734382

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Function(s)
GTPase activity protein binding GTP binding GTP-dependent protein binding thioesterase binding Rab GTPase binding enzyme binding protein kinase binding histone deacetylase binding Rho GDP-dissociation inhibitor binding protein kinase regulator activity calcium-dependent protein binding nucleotide binding
Process(es)
positive regulation of protein phosphorylation auditory receptor cell morphogenesis mast cell chemotaxis epithelial cell morphogenesis endocytosis phagocytosis, engulfment chemotaxis hyperosmotic response cytoskeleton organization actin filament organization cell adhesion G-protein coupled receptor signaling pathway small GTPase mediated signal transduction axon guidance metabolic process cell proliferation positive regulation of lamellipodium assembly regulation of fibroblast migration positive regulation of cell-substrate adhesion regulation of neuron maturation dendrite development cell migration cerebral cortex radially oriented cell migration embryonic olfactory bulb interneuron precursor migration cerebral cortex GABAergic interneuron development regulation of cell morphogenesis lamellipodium assembly actin cytoskeleton organization actin filament polymerization regulation of cell migration positive regulation of actin filament polymerization ruffle organization negative regulation of interleukin-23 production substrate adhesion-dependent cell spreading positive regulation of phosphatidylinositol 3-kinase activity engulfment of apoptotic cell cell-cell junction organization bone resorption positive regulation of DNA replication anatomical structure arrangement dendrite morphogenesis cell motility homeostasis of number of cells within a tissue positive regulation of stress fiber assembly localization within membrane positive regulation of focal adhesion assembly synaptic transmission, GABAergic Wnt signaling pathway, planar cell polarity pathway regulation of respiratory burst cellular response to mechanical stimulus semaphorin-plexin signaling pathway dopaminergic neuron differentiation protein localization to plasma membrane positive regulation of neutrophil chemotaxis cochlea morphogenesis ruffle assembly positive regulation of substrate adhesion-dependent cell spreading positive regulation of cell proliferation regulation of cell-substrate adhesion cell projection assembly lymphocyte aggregation regulation of neutrophil migration positive regulation of protein targeting to mitochondrion neuron projection development positive regulation of cell adhesion mediated by integrin intracellular signal transduction neuromuscular process controlling balance regulation of small GTPase mediated signal transduction signal transduction regulation of hydrogen peroxide metabolic process platelet activation regulation of T cell proliferation regulation of mast cell degranulation regulation of protein kinase activity regulation of mast cell chemotaxis movement of cell or subcellular component inflammatory response cell-matrix adhesion blood coagulation regulation of cell size response to wounding anatomical structure morphogenesis T cell costimulation positive regulation of Rho protein signal transduction Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of apoptotic process vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway negative regulation of receptor-mediated endocytosis regulation of defense response to virus by virus neuromuscular process Rac protein signal transduction
It has to be done as per old AB suggested Products section.
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